| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 33,456 | 0 | C | A | 53.8% | 2.7 / 12.9 | 13 | T880T (ACC→ACA) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base A (0/7); total (3/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCC > NZ_CP009273/33369‑33542 | gttcGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCaa > 2:245712/1‑90 (MQ=255) cccccGGCGTCCCGCGGGAAAAAGGGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCGGGGCGTACCCAAAGAAGTTATCccgc < 1:56323/87‑1 (MQ=255) ccGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCg < 2:100925/90‑1 (MQ=255) gCAAAAGGGGCGGCGCGCGTGATGGCGGGAAAATCGGTGGGGGAGGAGGGCGTAACCAAAGAATTTATCCCGCCGTACTACTCGGTGaaa < 1:336230/90‑1 (MQ=255) aaggTGGGGGCGGGGGTGACGGCTGGCAAACCGGTGGCTGAGCAGGGGGTACCAAAAGAAGTTCACCCGCCGTAATACTCGGTGAaagaa < 2:427705/87‑1 (MQ=255) gcggcgCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAgtggtg > 1:427705/1‑90 (MQ=255) gcggcgCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAgtggtg > 2:470682/1‑90 (MQ=255) cgcgcgTGATGGCGGCCAAATCGCGGGCTGAGCAGGGCGTAACAAAAAAATTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGtgctgc < 2:476030/90‑1 (MQ=255) aaaTCGCTGGCTGAGCGGGGCGTAACAAAAAAATTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCg < 2:456793/90‑1 (MQ=255) tggcggAGCAGGGCGTAACAAAAAAAGTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTg < 2:345440/90‑1 (MQ=255) ctgAGCAGGGCAAAACAAAAAAAGTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGAcc < 1:8224/90‑1 (MQ=255) tgAGCAGGGCGTAACAAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGAccc < 2:204380/90‑1 (MQ=255) aaCAAAAGAAGTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGcc < 2:46682/90‑1 (MQ=255) | GTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCC > NZ_CP009273/33369‑33542 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |