| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 349,999 | 0 | T | G | 60.0% | 0.6 / 12.8 | 15 | V528G (GTG→GGG) | prpE | propionate‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (9/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGG > NZ_CP009273/349917‑350064 | gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAAt > 2:436539/1‑90 (MQ=255) gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTTCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGCAAGATgcg < 1:426316/90‑1 (MQ=255) cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGGAAGGGGAggggg > 2:111651/1‑90 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAAAGGGCTGGAAGGGGAgggggg > 1:456800/1‑89 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGGGCTGAAAGGGCAgggggg > 2:399617/1‑89 (MQ=255) gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTc < 1:436539/90‑1 (MQ=255) gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTc < 2:97387/90‑1 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCAt > 1:160769/1‑90 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAACGGGCTGAAAGGGGGGGGGGGGGGGGGGTTTGTcct > 2:469121/1‑88 (MQ=255) aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCg > 2:11558/1‑90 (MQ=255) aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGTTTGTCATTCCGAAAGAGAGCg > 2:187683/1‑90 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:237160/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:258236/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:91882/90‑1 (MQ=255) ttGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGCGCTGAAAAGGGGGGGGGGGGGGGGGGTTGTTCTTTCGGAAGAGAGGGACAGGCTgg > 1:237160/1‑90 (MQ=255) | GCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGG > NZ_CP009273/349917‑350064 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |