| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,679,006 | 0 | A | C | 54.5% | 3.7 / 12.4 | 11 | D164A (GAC→GCC) | tus | DNA replication terminus site‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/6); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCC > NZ_CP009273/1678924‑1679095 | gcgGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGcc > 1:277395/1‑90 (MQ=255) gTTTTGAGGGGGTGCATCGTTTTTGGCGGGGGCTGACCCCCTTTATTCCTTCCCGCCCCCCCCCCTTTCTGCCCGCCCCCGCCACTTTAc < 1:497282/90‑1 (MQ=255) gtccATCGTCTTTGGCCGGGGCTGACCCCCTTTAATGCTTACCGCACGCTCACCTTTCTGCACGCCCCCGCCACTTTACGCTttggttgg < 2:167006/87‑1 (MQ=255) aTCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCta > 2:72036/1‑90 (MQ=255) gTCTTTGGCGGGGGCTAACCCCCTTTATTCCTTCCCGCCCGCTCCCCGTTCTGCACCCCCCCGCCACTTTACGCTTTGGTTGGGCtaata < 1:72036/90‑1 (MQ=255) tttAATGCTTCCCCCCCGCCCCCCTTTCTCCCCGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACAt < 1:316299/89‑1 (MQ=255) tAATGCTTCCCGCCCGCCCCCCTTTCTCCACGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCg < 2:212889/90‑1 (MQ=255) ttACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGtgatga > 2:247073/1‑90 (MQ=255) ttACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGtgatga > 2:271174/1‑90 (MQ=255) gCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTcc > 1:459233/1‑90 (MQ=255) cACGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGaaaaa < 1:338562/90‑1 (MQ=255) aCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCGGGCACAGCTGGAAAAAAGcc > 2:488090/1‑90 (MQ=255) | GCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCC > NZ_CP009273/1678924‑1679095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |