| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,567 | 0 | T | G | 60.0% | 8.2 / 13.6 | 15 | G81G (GGT→GGG) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (9/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCC > NZ_CP009273/2089489‑2089653 | cAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGtc < 1:410251/90‑1 (MQ=255) tGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAATCTCTggg > 2:470345/1‑89 (MQ=255) tGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAGTGTCTTGa > 1:402449/1‑90 (MQ=255) gtgtGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTGAATGTCTGGAAGATg > 1:236489/1‑90 (MQ=255) gtgtGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAGGCTCTGGgagacg > 2:245972/1‑88 (MQ=255) aGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAGTGTCTGGGAGAGGGcgc‑gga > 2:16780/1‑88 (MQ=255) aaaGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAGTTTCTGGAAGATGCCGCGGAAAAtt > 1:384896/1‑90 (MQ=255) tcGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGgcgc < 2:89404/90‑1 (MQ=255) gATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGGAGATGCCGC‑GAAAATTCTTTTCTTTTGCGCGGGTAAAAATTCCAt > 2:833/1‑90 (MQ=255) aTCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATc < 2:103613/90‑1 (MQ=255) tCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGGGTTTGGGAGATGCCCCGGAAAATTTTTTCTTTTGG‑GCGGGGAAAAATTtcatca > 1:255540/1‑90 (MQ=255) gACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAAc < 2:439416/90‑1 (MQ=255) aCATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTTTTTTCTTTTGCGCGGGTAAAAATTTCATCAAct > 2:54869/1‑90 (MQ=255) gtgtgGCGGGGGGGATTAAGTCTCTGGAAGAAGCCCC‑GAAAATTCTTTCCTTTTGCGGGGGTAAAAATTTCATCAACTCTCCTGCGCTgg > 1:469638/1‑90 (MQ=255) gggTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGAccc < 2:384896/90‑1 (MQ=255) | CAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGC‑GAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCC > NZ_CP009273/2089489‑2089653 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |