| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,247,849 | 0 | T | G | 56.2% | 14.5 / 17.4 | 16 | T273P (ACC→CCC) | rihB | ribosylpyrimidine nucleosidase |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (9/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATG > NZ_CP009273/2247762‑2247937 | cTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTAc < 2:425415/90‑1 (MQ=255) aaTCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGGTTTCCCAAAAAGCCCCGCCCGGCGCCGACGGGACGc > 1:55016/1‑90 (MQ=255) cccAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCAt < 1:383405/90‑1 (MQ=255) cAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGGTTGCCCAGAAAACCCAACGCGGCGCCGACGGGACGGCCCTAg > 1:356908/1‑90 (MQ=255) aGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAAAACGCCCAGCTCGTCGCAGACGGGACGCCCATAGCAAgggg > 2:208284/1‑89 (MQ=255) gTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCCAGAAAGCCCCGCCCGTCGCCGACGGGACGCCCCTAGCAAGGGCCAccg > 1:337889/1‑88 (MQ=255) gTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAAAGCCCAGCTCGGCGCAGACGGGACGCCCCTAGCAAGGGGCACTg > 2:455047/1‑90 (MQ=255) aTAGTGATGCCGACTTTGGTATTGGCGGGCTTTCCCAAAACGCCCCGCTCGTCGCAGACGGTACGCCCCTAGCAAGGGGCACTGGTGACg > 2:257717/1‑90 (MQ=255) tGATGCCGACTTTGGTATTGGCGGGGTTGCCCAAAACACCCACCTTCTCGCCGACGGGAAGCCCCTAGCAAAGGGCACTGTTGGCGTCCa > 1:18722/1‑90 (MQ=255) tGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCCGCTCGTCGCAGACGGGACGCCCCTAGCAAGGGGCCCTGTTGACGTccc > 2:338661/1‑89 (MQ=255) tGATGCCGACTTTGGTATTGGCGGGCTTGCCAAAAAAGCCCCGCTCGTCGCCGAAGGGAAGCCCCTAGGAAGGGGCCCTGTTGACGGccc > 1:84614/1‑89 (MQ=255) gATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGGACGCCCATAGCAAGGGGCACTGTTGACGTCCAc > 2:458575/1‑90 (MQ=255) tGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACtt < 2:356908/90‑1 (MQ=255) gCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTc < 1:208284/90‑1 (MQ=255) cgtcgCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCa > 2:176974/1‑90 (MQ=255) gTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATg < 2:74955/90‑1 (MQ=255) | CTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATG > NZ_CP009273/2247762‑2247937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |