| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,255,725 | 0 | A | C | 53.8% | 6.0 / 15.4 | 13 | G40G (GGT→GGG) | fruK | 1‑phosphofructokinase |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/7); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCC > NZ_CP009273/2255649‑2255809 | tGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCa > 2:429928/1‑90 (MQ=255) aGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGtt > 1:392839/1‑90 (MQ=255) gAAGCCCCCACCGGTAACACCAATTCCCGGGTTTTTTAATATTTGGCCCACGTTGATCCCTTTCCCCGCCGCATGCAGACCGGTGGtttt < 1:264980/90‑1 (MQ=255) ccgccAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCAcc > 1:90506/1‑90 (MQ=255) tCATTTCCCAGGTCTTTAAATTCTTTGCCCACGTTGATCCCTTTCCCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTcgccg < 1:300707/90‑1 (MQ=255) atccccAGGTTTTTTAATTCTTTGCCCCCTTTGTTCCTTTTCCCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGt < 1:246755/87‑1 (MQ=255) cTTTTAATACTTTGGACACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCg > 1:355297/1‑90 (MQ=255) tttGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAAcc > 1:61959/1‑90 (MQ=255) tttGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAAcc > 2:134295/1‑90 (MQ=255) ccACGTTGATGCCTTTCCCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACcaacaa < 2:213690/90‑1 (MQ=255) gATCCCTTTCCCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATa < 2:180117/90‑1 (MQ=255) cttttCCCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGcc < 1:199681/88‑1 (MQ=255) ccTTTCCCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGcc < 1:134295/90‑1 (MQ=255) | TGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCC > NZ_CP009273/2255649‑2255809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |