| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,569,307 | 0 | A | C | 57.9% | 18.0 / 21.0 | 19 | V372G (GTT→GGT) | gntU | gluconate transporter |
| Reads supporting (aligned to +/- strand): ref base A (4/4); new base C (0/11); total (4/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAG > NZ_CP009273/3569227‑3569393 | ggCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCa < 1:205177/90‑1 (MQ=255)ggCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCa < 1:340610/90‑1 (MQ=255) tGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCa < 2:81101/90‑1 (MQ=255) accGACCCCCCAGCAATAAAAACCGACAGCGCCGCCATTTGCCCCCCGGAGTAGTTCAGTTTTTAAATACCCGGCATCCCCAGTCCTAcc < 1:139523/88‑1 (MQ=255) ccaGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTaaa > 2:432788/1‑90 (MQ=255) ccaGCGATAAAAATCGACAGCGCCGCCTTTTGCGCCCCGGAGTAGTTCATTTGTTAAATACCCGGCATCCCCAGTCCTACCGCCGTTaaa < 1:149326/90‑1 (MQ=255) ccaGCGATAAAAACCGACAGCGCCGCCTTTGGCGCCCCGGAGTAGTTCAGTTGTTCAATACCCGGCATCACCAGTCCTACCGCCGTTaaa < 2:261892/90‑1 (MQ=255) caGCGATACAAACCGACAGCCCCGCCATTTGCGCCCCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAAc < 2:447357/90‑1 (MQ=255) aaccGACAGCGCCCCCTTTGGCCCCCGGGGGTAGTTCGGTTTTTCAAAACCCGGCACCCCCAGTCCTACCGCCGTTAAACAGGCTACGGt < 1:313913/87‑1 (MQ=255) ccccccgccTTTCGCCCACCGGAGTAGTTCAGTTGTTCAATACCCGCCCCCACCAGCCCTACCGCCGTTAAACAGGCTACGGTGGCAGaa < 1:67669/86‑1 (MQ=255) cgccTTTTCCCCCCCGGAGTATTTCAGTTGCCCAATACCCGCCACCCCCAGCCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTg < 1:63393/90‑1 (MQ=255) ccttttGCGCCCGGGAGTATTTCAGTTGTTAAATACCCGGCATCCCCAGTCCTCCCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGaa < 1:203492/87‑1 (MQ=255) ttggcgcCCGGGAGTATTTCAGTTGTTCAAAACCCGGCACCCCCAGTCCTCCCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAatga < 1:88209/87‑1 (MQ=255) ttGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAatgat > 1:378339/1‑90 (MQ=255) agtagtTCAGTTTTTCAAAACCCGGCATCCCCAGCCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAg < 2:116715/90‑1 (MQ=255) gtTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGc > 2:152046/1‑90 (MQ=255) cAATACCCGCCACCCCCAGCCCTCCCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGc < 1:210953/90‑1 (MQ=255) aaaTACCCGGCACCCCCAGCCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGc < 2:201921/89‑1 (MQ=255) aTAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAg > 2:97239/1‑90 (MQ=255) | GGCTGACAACAATCGAACCACCAGCGATACAAATCGACAGCGCCGCCATTTGCGCACCGGAGTAGTTCAGTTGTTCAATAACCGGCATCACCAGTCCTACCGCCGTTAAACAGGCTACGGTGGCAGAACCCTGAATGATGCGCACTGCAGCTGCCAGCACGAAGCAG > NZ_CP009273/3569227‑3569393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |