Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,527,9260AC54.5% 4.9 / 12.3 11G219G (GGT→GGG) nanMN‑acetylneuraminate epimerase
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base C (0/6);  total (4/7)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATC  >  NZ_CP009273/4527838‑4528006
                                                                                        |                                                                                  
ttAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCAc                                                                                   <  2:491811/90‑1 (MQ=255)
                  aaTCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATT‑CACAAccgccg                                                               >  1:429091/1‑90 (MQ=255)
                          tCAAATCCGGCACCCGTTCCCAACCCTGTTTGGGCTCCCCCATAAAAAACCCAGTTTTTATCCCCTTT‑ATC‑CACACCCGCCGCACCAGcc                                                       <  2:418425/90‑1 (MQ=255)
                                    ctcccGTCCGCATTCCTGTTTGGGCTCCCCAATAAAAAAGCCAGTTTTTATCCCTTTT‑ATT‑CAAACCCGCCGCACCAGCCGTTCCGTAcc                                             <  2:369970/87‑1 (MQ=255)
                                                 tCCTGGTTTGCTTTCCCCAATAA‑AAACCTGGTTTTACCCCCTTTAAAC‑CAAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc                                <  2:283087/90‑1 (MQ=255)
                                                       ttgggCTCCCCCTTTAATAAGCCGGGTTTTATCCCTTTT‑TTC‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTa                          <  2:129324/87‑1 (MQ=255)
                                                            cTTCGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTcc                     >  2:228939/1‑90 (MQ=255)
                                                                  ccataaaaaCCCCGGTTTTTACCCCTTT‑TTTCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGc                <  2:379553/87‑1 (MQ=255)
                                                                   aTTAAAAAGCCGGTTTTTATCCCCTTT‑ATC‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtg              <  2:27792/90‑1 (MQ=255)
                                                                             cAGGTTTTATCACCTTT‑ATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGa    >  2:475841/1‑90 (MQ=255)
                                                                               ggTTTTATCACCTTT‑ATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATc  >  2:63086/1‑90 (MQ=255)
                                                                                        |                                                                                  
TTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATC  >  NZ_CP009273/4527838‑4528006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑