Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,840,928	T→C	E97G (GAA→GGA)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,840,928	0	T	C	100.0%	28.7 / NA	10	E97G (GAA→GGA)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/5);  total (5/5)

CTTTTCCGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTC  >  NZ_CP009273/1840843‑1841007                                                                                      |                                                                                cTTTTCCGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCt                                                                             <  2:124053/90‑1 (MQ=255)    ttCCGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGc                                                                          >  2:428507/1‑90 (MQ=255)                        aGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTtga                                                      <  2:192609/90‑1 (MQ=255)                                   tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgt                                           >  1:236295/1‑90 (MQ=255)                                    cATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgtt                                          <  1:306488/90‑1 (MQ=255)                                       ccaccaGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgttgtt                                       <  2:86535/90‑1 (MQ=255)                                           caGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGt                                   >  2:450852/1‑90 (MQ=255)                                                           cGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGc                   <  2:336959/90‑1 (MQ=255)                                                             aTCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTg                 >  1:259650/1‑90 (MQ=255)                                                                            gTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc  >  2:209265/1‑90 (MQ=255)                                                                                      |                                                                                CTTTTCCGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTC  >  NZ_CP009273/1840843‑1841007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑