Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,061,342	T→A	intergenic (‑576/‑774)	BW25113_RS10415 ← / → BW25113_RS24005	IS5‑like element IS5 family transposase/TonB‑dependent receptor plug domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,061,342	0	T	A	100.0%	91.0 / NA	25	intergenic (‑576/‑774)	BW25113_RS10415/BW25113_RS24005	IS5‑like element IS5 family transposase/TonB‑dependent receptor plug domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/14);  total (11/14)

CTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCTAT  >  NZ_CP009273/2061267‑2061418                                                                            |                                                                             cTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTg                                                                <  1:16391/90‑1 (MQ=255)     tctcAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGaa                                                            <  2:234715/90‑1 (MQ=255)     tctcAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGaa                                                            <  1:305753/90‑1 (MQ=255)     tctcAAATTCTGCATCCTCAGGCACAGCTTTCTACTCCACGGTAATGGCCCAAATCCGAAATTGCTCAGGAACATTAGTGGTGCTGCGaa                                                            <  1:399078/90‑1 (MQ=255)                    tctcAGACACAGCTTTCTACTCCATGGTAAAGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGc                                             <  2:133262/90‑1 (MQ=255)                    tctcAGACACAGCCTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGc                                             <  1:415007/90‑1 (MQ=255)                     ctcAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCg                                            >  1:150875/1‑90 (MQ=255)                      tcAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCgg                                           >  1:481499/1‑90 (MQ=255)                      tcAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCgg                                           <  2:141675/90‑1 (MQ=255)                             caGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCa                                    >  2:180724/1‑90 (MQ=255)                             caGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCa                                    >  2:180723/1‑90 (MQ=255)                                        tCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCAc                         <  2:6616/90‑1 (MQ=255)                                                aaTGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTtat                 >  2:5513/1‑90 (MQ=255)                                                 aTGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTtata                >  2:453555/1‑90 (MQ=255)                                                 aTGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGGGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTtata                >  1:319360/1‑90 (MQ=255)                                                  tGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCCCTCGTCATCACGTATTtatat               <  1:453555/90‑1 (MQ=255)                                                   ggCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCgg                                           >  1:56403/1‑61 (MQ=255)                                                   ggCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCgg                                           <  2:56403/61‑1 (MQ=255)                                                   ggCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTtatata              >  1:374265/1‑90 (MQ=255)                                                       cAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCAt          <  2:300896/90‑1 (MQ=255)                                                        aaaTACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATg         >  1:20337/1‑90 (MQ=255)                                                        aaaTACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATg         >  2:260977/1‑90 (MQ=255)                                                          aTACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGaa       <  2:178074/90‑1 (MQ=255)                                                          aTACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGaa       <  1:136012/90‑1 (MQ=255)                                                               aaGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCTAt  <  2:252956/90‑1 (MQ=255)                                                                            |                                                                             CTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCTAT  >  NZ_CP009273/2061267‑2061418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑