Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,202,478	T→G	Y345D (TAT→GAT)	yehP →	VWA domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,202,478	0	T	G	100.0%	36.9 / NA	13	Y345D (TAT→GAT)	yehP	VWA domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/5);  total (8/5)

GACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCAT  >  NZ_CP009273/2202396‑2202553                                                                                   |                                                                            gACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCGATGACcg                                                                      >  1:343404/1‑90 (MQ=255) gACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCGATGACcg                                                                      >  1:96960/1‑90 (MQ=255)       tCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCGATGACCGCGATAc                                                                <  2:178835/90‑1 (MQ=255)             gAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCGATGACCGCGATACGGCCCa                                                          <  1:58874/90‑1 (MQ=255)               aaaaGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCGATGACCGCGATACGGCCCAgg                                                        >  2:43453/1‑90 (MQ=255)                  aGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCGATGACCGCGATACGGCCCAGgcgc                                                     <  1:285447/90‑1 (MQ=255)                                           gCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCGATGACCGCGATACGGCCCAGgcgc                                                     >  1:149759/1‑65 (MQ=255)                                           gCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCGATGACCGCGATACGGCCCAGgcgc                                                     <  2:149759/65‑1 (MQ=255)                                                                  gCACCGCAACACCTTGCGATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTgg     >  2:448051/1‑90 (MQ=255)                                                                     ccGCAACACCTTGCGATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGcat  >  1:435712/1‑90 (MQ=255)                                                                     ccGCAACACCTTGCGATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGcat  >  2:215346/1‑90 (MQ=255)                                                                               ttGCGATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAg                             >  1:57390/1‑53 (MQ=255)                                                                               ttGCGATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAg                             <  2:57390/53‑1 (MQ=255)                                                                                   |                                                                            GACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCAT  >  NZ_CP009273/2202396‑2202553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑