Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,375,251	A→G	G55G (GGA→GGG)	rbn →	ribonuclease BN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,375,251	0	A	G	100.0%	62.0 / NA	20	G55G (GGA→GGG)	rbn	ribonuclease BN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/12);  total (8/12)

CAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCA  >  NZ_CP009273/2375165‑2375335                                                                                       |                                                                                     cAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGgaaa                                                                                   >  1:443057/1‑90 (MQ=255) cAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGgaaa                                                                                   >  1:55814/1‑90 (MQ=255)  aaCATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAAc                                                                                  <  2:335764/90‑1 (MQ=255)       ccGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGAc                                                                             <  1:491995/90‑1 (MQ=255)         gACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACaa                                                                           <  1:502726/90‑1 (MQ=255)               gagCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGAtttt                                                                     >  2:414725/1‑90 (MQ=255)                    gACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCa                                                                >  1:194109/1‑90 (MQ=255)                       tttGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTc                                                             <  1:87016/90‑1 (MQ=255)                       tttGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTc                                                             <  2:47382/90‑1 (MQ=255)                       tttGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTc                                                             <  2:469517/90‑1 (MQ=255)                             tgttTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCCGTCACCTTc                                                       >  1:347208/1‑90 (MQ=255)                              gttTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCa                                                      >  1:3119/1‑90 (MQ=255)                                      gCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGatc                                              >  1:6632/1‑90 (MQ=255)                                                  cccAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGtt                                  >  1:298589/1‑90 (MQ=255)                                                      gCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTAcc                              <  2:134903/90‑1 (MQ=255)                                                           aGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCt                         <  2:298589/90‑1 (MQ=255)                                                           aGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCt                         <  2:444316/90‑1 (MQ=255)                                                           aGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCt                         <  2:54063/90‑1 (MQ=255)                                                                    aTACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGAGCATCTTTTTGGTTTACCCGGCTTGCTGTGCa                >  1:351649/1‑90 (MQ=255)                                                                      aCCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGt              <  2:188747/90‑1 (MQ=255)                                                                            tttAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCt        >  1:174048/1‑90 (MQ=255)                                                                                  ccTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCa  <  2:471288/90‑1 (MQ=255)                                                                                       |                                                                                     CAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCA  >  NZ_CP009273/2375165‑2375335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑