Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,434,529	A→C	F186V (TTT→GTT)	fabB ←	beta‑ketoacyl‑ACP synthase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,434,529	0	A	C	100.0%	49.1 / NA	16	F186V (TTT→GTT)	fabB	beta‑ketoacyl‑ACP synthase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (5/11);  total (5/11)

AGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCAC  >  NZ_CP009273/2434458‑2434597                                                                        |                                                                     agtagACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGcc                                                    <  1:264435/90‑1 (MQ=255)       cAGCGCACCCATTGCGCCGAATTCGCAAGCCATTTCCCAGCCCAGCTCTTCCCCGCCCCCAGCACCCACGATGCCCTGTTTGCCCAGTTg                                              >  1:200339/1‑90 (MQ=255)         gcgcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGa                                            >  1:227953/1‑90 (MQ=255)         gcgcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGa                                            <  2:107289/90‑1 (MQ=255)         gcgcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGa                                            <  2:211457/90‑1 (MQ=255)           gcccccATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATc                                          <  2:81806/87‑1 (MQ=255)           gcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATc                                          <  2:230012/90‑1 (MQ=255)                   tGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTAc                                  <  2:158252/90‑1 (MQ=255)                       tCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAGCCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCg                              <  1:362561/90‑1 (MQ=255)                         gAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGtt                            <  2:129614/90‑1 (MQ=255)                                 cacccATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATAc                    <  1:306437/87‑1 (MQ=255)                                 aaGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATAc                    <  1:363703/90‑1 (MQ=255)                                                   gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATAc                    >  1:406163/1‑72 (MQ=255)                                                   gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATAc                    <  2:406163/72‑1 (MQ=255)                                                   gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGcac  >  1:487159/1‑90 (MQ=255)                                                   gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGcac  >  2:188656/1‑90 (MQ=255)                                                                        |                                                                     AGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCAC  >  NZ_CP009273/2434458‑2434597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑