Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,526,050	A→G	T95A (ACC→GCC)	cysK →	cysteine synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,526,050	0	A	G	100.0%	20.4 / NA	8	T95A (ACC→GCC)	cysK	cysteine synthase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/3);  total (5/3)

AACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGAACGCCGCAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACGGAAGGTGCTAAAG  >  NZ_CP009273/2525967‑2526122                                                                                    |                                                                         aaCCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAGCCATGa                                                                    >  1:132031/1‑90 (MQ=255)     gACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAGCCATGAGTAt                                                                <  2:90438/90‑1 (MQ=255)                    cGGGATTGCACTGGCCTATGTAGTTGCCGCTCGCGGTTACAAACTCGCCCTGACCATGCCAGAAGCCAGGAGTATTGAACGCCGCGAgct                                                 >  1:83203/1‑90 (MQ=255)                            cACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAGCCATGAGTATTGAACGCCGCAAGCTGCTGAAAg                                         <  1:170960/90‑1 (MQ=255)                                             gCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAGCCATGAGTATTGAACGCCGCAAgc                                                  <  1:94970/64‑1 (MQ=255)                                             gCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAGCCATGAGTATTGAACGCCGCAAgc                                                  >  2:94970/1‑64 (MQ=255)                                               cGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAGCCATGAGTATTGAACGCCGCAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGt                      >  1:277717/1‑90 (MQ=255)                                                                   cccTGACCATGCCAGAAGCCATGAGTATTGAACGCCGCAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACGGAAGGTGCTAAAg  >  1:38720/1‑90 (MQ=255)                                                                                    |                                                                         AACCGACCAGCGGTAATACCGGGATTGCACTGGCCTATGTAGCTGCCGCTCGCGGTTACAAACTCACCCTGACCATGCCAGAAACCATGAGTATTGAACGCCGCAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACGGAAGGTGCTAAAG  >  NZ_CP009273/2525967‑2526122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑