Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,751,237	A→G	V241V (GTT→GTC)	BW25113_RS13685 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,751,237	0	A	G	100.0%	73.9 / NA	23	V241V (GTT→GTC)	BW25113_RS13685	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/13);  total (10/13)

CAAACTCTGACATTTGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCCTTCTTT  >  NZ_CP009273/2751152‑2751315                                                                                      |                                                                               cAAACTCTGACATTTGTATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACag                                                                            <  1:356890/90‑1 (MQ=255)   aaCTCTGACATTTGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACagag                                                                          >  1:476031/1‑90 (MQ=255)       ctGACATTTGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCat                                                                      >  2:43254/1‑90 (MQ=255)       ctGACATTTGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCat                                                                      >  1:336832/1‑90 (MQ=255)        tGACATTTGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCatt                                                                     >  2:51331/1‑90 (MQ=255)                    aaaaTCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCACTCGGGACAGAGTCATTAATTAGCTcaac                                                         <  2:122486/90‑1 (MQ=255)                              aGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGt                                               <  1:460153/90‑1 (MQ=255)                                 ggTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGa                                            >  2:464124/1‑90 (MQ=255)                                  gTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACag                                                                            <  1:161520/57‑1 (MQ=255)                                  gTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACag                                                                            >  2:161520/1‑57 (MQ=255)                                   ttCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAAt                                          <  1:424266/90‑1 (MQ=255)                                        tAAGTTCATTAACTGCTACAACCTTATTTCTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGatat                                     <  2:272301/90‑1 (MQ=255)                                                    cTGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCg                         >  1:90921/1‑90 (MQ=255)                                                     tGCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCgc                        >  1:312507/1‑90 (MQ=255)                                                      gCTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCgct                       >  1:302707/1‑90 (MQ=255)                                                       cTACAACCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCgctt                      <  2:160074/90‑1 (MQ=255)                                                           aaCCTTATTTTTCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCgctttgct                  <  2:300479/90‑1 (MQ=255)                                                                   ttttCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCAc          <  1:264289/90‑1 (MQ=255)                                                                     ttCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCAccc        <  2:339778/90‑1 (MQ=255)                                                                      tCCACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCct       <  2:336832/90‑1 (MQ=255)                                                                        cACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCcttc     <  1:51331/90‑1 (MQ=255)                                                                        cACCCATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCcttc     <  1:464124/90‑1 (MQ=255)                                                                           ccATCATTCGGGACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCCTTCttt  >  1:196214/1‑90 (MQ=255)                                                                                      |                                                                               CAAACTCTGACATTTGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCCTTCTTT  >  NZ_CP009273/2751152‑2751315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑