Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,728,024	A→C	S315S (TCA→TCC)	xylH →	xylose ABC transporter permease XylH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,728,024	0	A	C	100.0%	88.5 / NA	27	S315S (TCA→TCC)	xylH	xylose ABC transporter permease XylH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/13);  total (14/13)

CACCAAACTTGCCGTGTTCGCGATTAACGGATTAATGGTAGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCACCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATGCGTGATTGGCGGCACCAGCCTGGCTGGCGGTGTGGGAAGC  >  NZ_CP009273/3727940‑3728108                                                                                     |                                                                                     cACCAAACTTGCCGTGTTCGCGATTAACGGATTAATGGTAGCCATCGCCGGATTAATCCTTAGTTCTCGACTTTGCGCTGGTTCCCCTTc                                                                                 >  1:118327/1‑90 (MQ=255) cACCAAACTTGCCGTGTTCGCGATTAACGGATTAATGGTAGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTc                                                                                 <  1:335462/90‑1 (MQ=255)      aaCTTGCCGTGTTCGCGATTAACGGATTAATGGTAGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCgg                                                                            <  1:196279/90‑1 (MQ=255)           gCCGTGTTCGCGATTAACGGATTAATGGTAGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAat                                                                       >  2:344967/1‑90 (MQ=255)                  tCGCGATTAACGGATTAATGGTAGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCg                                                                >  2:78444/1‑90 (MQ=255)                          aaCGGATTAATGGTAGCCATCGCCGGATTAATCCTTAGTTCTCGACTTTGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGAc                                                        <  2:118327/90‑1 (MQ=255)                          aaCGGATTAATGGTAGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGAc                                                        <  2:489302/90‑1 (MQ=255)                          aaCGGATTAATGGTAGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGAc                                                        >  2:136514/1‑90 (MQ=255)                          aaCGGATTAATGGTAGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGAc                                                        <  1:384071/90‑1 (MQ=255)                          aaCGGATTAATGGTAGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGAc                                                        <  2:1104/90‑1 (MQ=255)                               aTTAATGGTAGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAAt                                                   >  2:332604/1‑90 (MQ=255)                                    tggtggCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGATCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTgcag                                              <  2:445628/90‑1 (MQ=255)                                        aGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATg                                          >  1:454317/1‑90 (MQ=255)                                        aGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATg                                          >  1:344313/1‑90 (MQ=255)                                        aGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATg                                          >  1:323847/1‑90 (MQ=255)                                        aGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATg                                          >  2:86450/1‑90 (MQ=255)                                            aTCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATGCGTg                                      >  1:362968/1‑90 (MQ=255)                                              cGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATGCGTGAt                                    <  1:455785/90‑1 (MQ=255)                                                       aaTCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATGCGTGATTGGCGGCAc                           <  1:78444/90‑1 (MQ=255)                                                       aaTCCTTAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATGCGTGATTGGCGGCAc                           <  2:344313/90‑1 (MQ=255)                                                             tAGTTCTCGACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGAAATTGCAGCATGCGTGATTGGCGGCAACAGTct                     >  1:235391/1‑90 (MQ=255)                                                                     gACTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATGCGTGATTGGCGGCACCAGCCTGGCTGGCg             <  1:154093/90‑1 (MQ=255)                                                                      aCTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATGCGTGATTGGCGGCACCAGCCTGGCTGGCgg            <  2:85664/90‑1 (MQ=255)                                                                       cTTGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATGCGTGATTGGCGGCACCAGCCTGGCTGGCGgt           <  2:320578/90‑1 (MQ=255)                                                                         tGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATGCGTGGTTGGCGGCACCAGCCTGGCTGGCGgtgt         >  1:424906/1‑90 (MQ=255)                                                                         tGGCGCTGGTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATGCGTGATTGGCGGCACCAGCCTGGCTGGCGgtgt         >  1:141397/1‑90 (MQ=255)                                                                                ggTTCCCCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATGCGTGATTGGCGGCACCAGCCTGGCTGGCGGTGTGGGAAGc  >  2:231545/1‑90 (MQ=255)                                                                                     |                                                                                     CACCAAACTTGCCGTGTTCGCGATTAACGGATTAATGGTAGCCATCGCCGGATTAATCCTTAGTTCTCGACTTGGCGCTGGTTCACCTTCTGCGGGAAATATCGCCGAACTGGACGCAATTGCAGCATGCGTGATTGGCGGCACCAGCCTGGCTGGCGGTGTGGGAAGC  >  NZ_CP009273/3727940‑3728108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑