Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,153,816	T→C	S84P (TCA→CCA)	btuB →	TonB‑dependent vitamin B12 receptor BtuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,153,816	0	T	C	100.0%	32.4 / NA	12	S84P (TCA→CCA)	btuB	TonB‑dependent vitamin B12 receptor BtuB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/7);  total (5/7)

CGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGG  >  NZ_CP009273/4153742‑4153892                                                                           |                                                                             cGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTc                                                               <  2:62494/90‑1 (MQ=255)   aCCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTcgc                                                             <  2:401680/90‑1 (MQ=255)       cGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGGTCAGGGCAGCTCCCATCTATTTTTATTTCCGGTa                                                         >  1:354514/1‑90 (MQ=255)         gTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCCCCCATCTATTTTTTTTCGCGGGACa                                                       >  1:319143/1‑90 (MQ=255)              tgatgTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGc                                                  <  1:454923/90‑1 (MQ=255)               gatgTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGcc                                                 <  2:382263/90‑1 (MQ=255)                         gcCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATgtgt                                       <  2:93221/90‑1 (MQ=255)                                 ccGGGCGTCGATATCACCCAAAACGGCGGTTCCGGGCAGCTCCCCTCTATTTTTTTTTTCCGGACAAAAGCCAGTCATGTGTTGGGGTTa                               >  1:410528/1‑90 (MQ=255)                                      cGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGGACAAATGCCAGGCATGTGTTGGGGTTAATTGa                          >  2:492118/1‑90 (MQ=255)                                            tagcACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGt                    <  1:269484/87‑1 (MQ=255)                                                          gcggTTCAGGTCAGCTCCCATCTATTTTTTTTTGCGGTACAAATGCCAGTCATGTGTTGGGGTTTATTGATGGCGTACGCCTGAATCTgg      >  1:128756/1‑90 (MQ=255)                                                              ttCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCggg  <  1:37988/90‑1 (MQ=255)                                                                           |                                                                             CGACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGG  >  NZ_CP009273/4153742‑4153892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑