| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 56,392 | A→C | Y240D (TAC→GAC) | lptD ← | LPS assembly protein LptD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 56,392 | 0 | A | C | 97.1% | 90.2 / NA | 35 | Y240D (TAC→GAC) | lptD | LPS assembly protein LptD |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (20/14); minor base G (1/0); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGC > NZ_CP009273/56306‑56472 | gCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTCGtt < 2:50438/90‑1 (MQ=255)gCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTCGtt > 1:50438/1‑90 (MQ=255) gaTGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGGAGAAATCCAAATGGTtgggggggg > 1:292938/1‑90 (MQ=255) aTGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGGAGAACTCAAAGTCGTtggtgggggg > 2:137167/1‑89 (MQ=255) aTGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGGAGAAATCAAAATCGTTggtggtggt > 1:492678/1‑90 (MQ=255) taATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGGAGAACTCAAAGTCGTTGGTGGTGGGGTACTTg > 2:130662/1‑90 (MQ=255) ggcgTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGGAGAACTCAAAGTCGTTGGTGGGGGGGTACTTGGCGTTc > 2:75422/1‑90 (MQ=255) gcgTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGGAGAACTCAAAATCGTTGGTGGGGGGGTACTTGGCGGTCg > 2:321547/1‑90 (MQ=255) gcgTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGGAGAAATCAAAGGCGTTGGGGGGGGGGGAATTTGCGtttg > 2:400709/1‑88 (MQ=255) cgTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGGAGAACTCAAAAGCGTTGGGGGGGGGGGACTTGGCGTTCgg > 1:60753/1‑90 (MQ=255) gTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCggg > 2:133327/1‑90 (MQ=255) tGATGGTGGCATCCATATTTGGCACGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGa < 1:73593/90‑1 (MQ=255) gtggCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAg < 1:133327/90‑1 (MQ=255) ggCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGaa < 1:400709/90‑1 (MQ=255) ggCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAAATCAAAGTCGTTGGTGGGGGGGTACTTGGGGGTCGGGATCAAGaa > 2:264269/1‑90 (MQ=255) ggCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGGAGAAATCAAAATCGTTGGGGGGGGGGTACTTGGGGTTCGGGGTAAAGaa > 1:267952/1‑90 (MQ=255) atTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCg < 1:82490/90‑1 (MQ=255) tttGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGa < 1:352888/90‑1 (MQ=255) tttGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTCGTTGGTGGTGGGGTACTTGGCGGTCGGGGTCAAGAAAACAGAGCGa > 2:490829/1‑90 (MQ=255) tGGCGCGATGTTCCAGTAATATGGCAGGGAGAACTCAAAAACGTTTGGGGGGGGGTTCTTGGCGTTCGGGGTCCAAAAAACAGAgcggcg > 1:444084/1‑90 (MQ=255) gcgcGATGTTCCAGTAATATGGCAGGGAGAACTCAAAGTCGTTGGTGGTGGGGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGtt > 1:54813/1‑90 (MQ=255) gcgcGATGTTCCAGTAATATGGCAGGGAGAACTCAAAGTCGTTGGGGGTGGGGTACTTGGCGGTCGGGATCAAGAAAACAGAGCGACGtt > 1:44944/1‑90 (MQ=255) gATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTc > 2:171518/1‑90 (MQ=255) gATGTTCCAGTAATATGGCAGGGAGAAATCAAAGTCGTTGGGGGGGGGGTGCTTGGCGTTCGGGGTCAAGAAACCAGAGCGGCGGTTGtt > 1:15007/1‑89 (MQ=255) aTGTTCCAGTAATATGGCAGGGAGAACTCAAAGTCGTTGGTGGGGGGGTACTTGGCGTTCGGGGTCAAGAAAACAGAGCGACGTTTGTca > 1:35928/1‑90 (MQ=255) gTTCCAGTAATATGGCAGGTAGAACTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTcacc < 2:484481/90‑1 (MQ=255) ccAGTAATATGGCAGGTAGAACTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTcacccac < 2:292938/90‑1 (MQ=255) tAATATGGCAGGTAGAACTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGc < 2:444084/90‑1 (MQ=255) aaTATGGCAGGTAGAACTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCa < 1:360529/90‑1 (MQ=255) aaTATGGCAGGTAGAACTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCa < 1:137167/90‑1 (MQ=255) atatGGCAGGTAGAACTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCaa < 1:321547/90‑1 (MQ=255) aGGTAGAACTCAAAGTCGTTGGTGGGGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCaaa > 1:149842/1‑90 (MQ=255) tAGAACTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAg < 1:101220/90‑1 (MQ=255) aCTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGc > 1:321297/1‑90 (MQ=255) aCTCAAAGTCGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGc < 2:35928/90‑1 (MQ=255) | GCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATCAAGAAACCAGAGCGACGTTTGTCACCCACCGGCAACTGCAAATAGGGGC > NZ_CP009273/56306‑56472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |