Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,394,663	T→C	G456G (GGA→GGG)	abgT ←	p‑aminobenzoyl‑glutamate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,394,663	0	T	C	100.0%	39.2 / NA	13	G456G (GGA→GGG)	abgT	p‑aminobenzoyl‑glutamate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/3);  total (10/3)

AGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAA  >  NZ_CP009273/1394608‑1394731                                                        |                                                                     aGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGgcg                                    >  2:20743/1‑90 (MQ=255) aGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGgcg                                    >  2:92243/1‑90 (MQ=255)           aTAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCaa                          <  2:187301/90‑1 (MQ=255)                aagtaCCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACgg                     >  1:361149/1‑90 (MQ=255)                    aCCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGa                 >  1:272630/1‑90 (MQ=255)                        aGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCg             >  1:438212/1‑90 (MQ=255)                        aGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCg             >  2:394360/1‑90 (MQ=255)                          tttCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGc           <  1:126129/90‑1 (MQ=255)                            tCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCaa         >  1:497210/1‑90 (MQ=255)                            tCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCaa         >  2:436130/1‑90 (MQ=255)                                   tGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGaaa  >  1:379636/1‑90 (MQ=255)                                                 gCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGc           <  1:299344/67‑1 (MQ=255)                                                 gCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGc           >  2:299344/1‑67 (MQ=255)                                                        |                                                                     AGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAA  >  NZ_CP009273/1394608‑1394731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑