Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,434,377	A→G	F222S (TTC→TCC)	nifJ ←	pyruvate:ferredoxin (flavodoxin) oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,434,377	0	A	G	100.0%	39.8 / NA	13	F222S (TTC→TCC)	nifJ	pyruvate:ferredoxin (flavodoxin) oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/4);  total (9/4)

CAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGA  >  NZ_CP009273/1434300‑1434445                                                                              |                                                                     cAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAg                                                          >  2:72083/1‑90 (MQ=255)              catCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGt                                             <  1:492926/90‑1 (MQ=255)               atCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTa                                            >  2:471143/1‑90 (MQ=255)                                gTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATg                           >  2:409138/1‑90 (MQ=255)                                          gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGa                             >  1:454266/1‑78 (MQ=255)                                          gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGa                             <  2:454266/78‑1 (MQ=255)                                          gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                 <  1:321655/90‑1 (MQ=255)                                          gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                 >  1:405045/1‑90 (MQ=255)                                          gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                 >  1:83874/1‑90 (MQ=255)                                          gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                 >  2:312000/1‑90 (MQ=255)                                                tACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGcc           >  2:44580/1‑90 (MQ=255)                                                       ggTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGgcgc    <  1:320721/90‑1 (MQ=255)                                                         ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGa  >  2:365300/1‑90 (MQ=255)                                                                              |                                                                     CAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGA  >  NZ_CP009273/1434300‑1434445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑