Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,502,076	A→G	N347D (AAC→GAC)	rlhA →	23S rRNA 5‑hydroxycytidine C2501 synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,502,076	0	A	G	100.0%	18.2 / NA	7	N347D (AAC→GAC)	rlhA	23S rRNA 5‑hydroxycytidine C2501 synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/3);  total (4/3)

AGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCA  >  NZ_CP009273/1502006‑1502160                                                                       |                                                                                     agtagtGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTCCCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGa                                                                   <  1:454260/90‑1 (MQ=255)            tGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGtt                                                        >  2:374310/1‑90 (MQ=255)               cGAAAGATCATCTCGTTGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTc                                                     <  1:316000/90‑1 (MQ=255)                              aTGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCg                                      >  2:39037/1‑90 (MQ=255)                                  tGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGaa                                  >  2:92798/1‑90 (MQ=255)                                                cACTGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATc                    >  2:295100/1‑90 (MQ=255)                                                                  gCCTGGACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCa  <  2:143521/90‑1 (MQ=255)                                                                       |                                                                                     AGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCA  >  NZ_CP009273/1502006‑1502160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑