Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,560,820	T→C	E193G (GAA→GGA)	dosC ←	diguanylate cyclase DosC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,560,820	0	T	C	100.0%	31.9 / NA	11	E193G (GAA→GGA)	dosC	diguanylate cyclase DosC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/5);  total (6/5)

AACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTTCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCT  >  NZ_CP009273/1560736‑1560903                                                                                     |                                                                                    aaCCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCAt                                                                                <  1:210621/90‑1 (MQ=255)    cacaGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGaa                                                                             <  1:229520/90‑1 (MQ=255)                       ggCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGcc                                                          >  1:318223/1‑90 (MQ=255)                       ggCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGcc                                                          >  2:169948/1‑90 (MQ=255)                                 cAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTttc                                                >  2:328421/1‑90 (MQ=255)                                  aaaCTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTttct                                               >  2:37086/1‑90 (MQ=255)                                          gCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGc                                       >  2:240867/1‑90 (MQ=255)                                                                    tataGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGAt             <  1:238717/90‑1 (MQ=255)                                                                        gATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGtt         >  2:483549/1‑90 (MQ=255)                                                                               tCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCt  <  2:352561/90‑1 (MQ=255)                                                                                  aTTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGagaa                     <  1:448640/68‑1 (MQ=255)                                                                                     |                                                                                    AACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTTCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCT  >  NZ_CP009273/1560736‑1560903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑