Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,568,198	A→G	F417S (TTC→TCC)	pqqL ←	insulinase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,568,198	0	A	G	100.0%	36.0 / NA	12	F417S (TTC→TCC)	pqqL	insulinase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/7);  total (5/7)

GGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTT  >  NZ_CP009273/1568143‑1568282                                                        |                                                                                     ggTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCg                                                    >  2:298536/1‑90 (MQ=255)  gTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGa                                                   >  2:15429/1‑90 (MQ=255)   tAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGAc                                                  <  1:204408/90‑1 (MQ=255)       ttGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGt                                              <  1:26592/90‑1 (MQ=255)       ttGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGt                                              <  2:357274/90‑1 (MQ=255)          ctgcCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAg                                           >  2:140885/1‑90 (MQ=255)                  gACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTa                                   <  1:243818/90‑1 (MQ=255)                      tttCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATc                               <  1:140885/90‑1 (MQ=255)                      tttCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATc                               <  1:385653/90‑1 (MQ=255)                                           ttCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAAc          >  1:107531/1‑90 (MQ=255)                                            tCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAAcc         >  1:68150/1‑90 (MQ=255)                                                   aCAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCAtttt  <  1:250534/90‑1 (MQ=255)                                                        |                                                                                     GGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTT  >  NZ_CP009273/1568143‑1568282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑