Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,576,388	A→G	S132P (TCC→CCC)	ydeN ←	sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,576,388	0	A	G	100.0%	24.9 / NA	9	S132P (TCC→CCC)	ydeN	sulfatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/1);  total (8/1)

GAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGCAG  >  NZ_CP009273/1576313‑1576471                                                                            |                                                                                    gAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCca                                                                       >  1:48085/1‑90 (MQ=255)          tATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGt                                                              <  1:325268/90‑1 (MQ=255)                   aGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAAc                                                     >  1:188568/1‑90 (MQ=255)                   aGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAAc                                                     >  1:209403/1‑90 (MQ=255)                                               ggTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAg                         >  1:318918/1‑90 (MQ=255)                                               ggTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAg                         >  1:502446/1‑90 (MQ=255)                                               ggTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAg                         >  2:89224/1‑90 (MQ=255)                                                              gcgGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGAttt          >  1:141468/1‑90 (MQ=255)                                                                      ggCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGCAg  >  2:20483/1‑90 (MQ=255)                                                                            |                                                                                    GAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGCAG  >  NZ_CP009273/1576313‑1576471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑