| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,673,542 | A→G | K287E (AAG→GAG) | ydgH → | DUF1471 family protein YdgH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,673,542 | 0 | A | G | 93.8% | 42.3 / NA | 16 | K287E (AAG→GAG) | ydgH | DUF1471 family protein YdgH |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (4/11); total (4/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCG > NZ_CP009273/1673458‑1673611 | atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa < 1:74284/90‑1 (MQ=255) cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa > 1:164116/1‑90 (MQ=255) cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa < 2:103222/90‑1 (MQ=255) cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa < 2:55838/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTCCGGCAACATGACCGAAGTCTCCTATCAGGTTGCTAAACGTGCCGCGGAGAAAGGTGCTaa < 1:312073/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa < 1:207296/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa < 1:220869/90‑1 (MQ=255) ttCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAg < 1:230495/90‑1 (MQ=255) aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTAtt < 2:164116/90‑1 (MQ=255) ctGGCAACTACGGCAACATGACCGAAGTCTCCTGTCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGcc > 1:493503/1‑90 (MQ=255) ctGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCt > 1:197195/1‑68 (MQ=255) ctGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCt < 2:197195/68‑1 (MQ=255) aaCATGCCCGAAGTCTCCAATCAGGTTGCAAAACGTGCCGCGGAAAAAGGTGCAAATTATTCCCACATCCCCCGCCAGTGGCAGGAACGt < 1:56226/90‑1 (MQ=255) aaCATGCCAGAAGCCTCCTACAAGGTGGCAAACGGTGGCGGGAAGAAAGGTACTAATTATTCCCACACCCCCCGCCAGTGGCAGGAACGt < 1:445309/90‑1 (MQ=255) tATCGGGTTGCGAACCGTGCCGGGGAGAAAGGTGCTAAGTTTTACCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc < 2:118417/90‑1 (MQ=255) aGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcg > 2:190732/1‑90 (MQ=255) | ATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCG > NZ_CP009273/1673458‑1673611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |