| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 5,159 | 0 | G | A | 50.0% | 5.4 / 10.8 | 18 | intergenic (+139/‑75) | thrC/yaaX | threonine synthase/DUF2502 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (9/0); new base A (0/9); total (9/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGA > NZ_CP009273/5079‑5243 | gaagaaATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTTTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATaacaac > 1:283386/1‑90 (MQ=255)gaagaaATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATaacaac > 2:703101/1‑90 (MQ=255) aaaaTTAGGGAAAAAAATGAGGGGAAAAAAGGAAAAATTCTCAATAAATGGGGTAACTGAAAGATTAGGTTGGCGGAAAATAACAAccgc < 1:555388/89‑1 (MQ=255) ttAGGGAAAAAAATGACAGGAAAAAAGGAAAAATTCTCAATAAAGGCGGTAACTTAGAGATTAGGATTGCGGAAAATAACAACCGCCGtt < 1:2277/90‑1 (MQ=255) gaAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCg > 1:43229/1‑90 (MQ=255) aaTGACAGGAAAAAAGGAAAAATTCTAAAAAAAGGGGGTAACTTAGAGATTAGGATTGGGGAAAATAACAACCGCCGTTCTCATCGAGTa < 1:915001/90‑1 (MQ=255) gAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGa > 1:393322/1‑90 (MQ=255) aaaGAAAAATTTCTAAAAAAAGGGGGAAATTAAGAGATTAGGATGGGGGAAAAAACCAACCGCCGTTCTCATCGAGTAATCTCCGGatat < 1:703101/90‑1 (MQ=255) aaGGAGAAATTCTAAATAAATGCGGTAACTTAGAGATTAGGTTTGCGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATc < 1:407647/90‑1 (MQ=255) aaGGAAAAATCCTAAAAAAATGGGTTAACTTAGAAATTAGGATTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATc < 1:454894/90‑1 (MQ=255) ttCTAAAAAAAGGCGTTAATTAAGAAATTAGGATGGCGAAAAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATaa < 2:14036/90‑1 (MQ=255) tcAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACgg > 1:27149/1‑90 (MQ=255) aaaaTGCGGTAATATAGAAATTAGGATGGGGAAAAAAAAAACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCaa < 2:723395/89‑1 (MQ=255) ggTAATTAAGAGATAAGGATGGGGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaaa < 2:27149/90‑1 (MQ=255) ttAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgt > 1:847074/1‑90 (MQ=255) ttAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgt > 1:696334/1‑90 (MQ=255) aGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGa > 1:555850/1‑90 (MQ=255) gCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGa > 1:221927/1‑90 (MQ=255) | GAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGA > NZ_CP009273/5079‑5243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |