| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 353,601 | 0 | C | A | 67.7% | 7.3 / 22.8 | 31 | L267F (TTG→TTT) | cynR | transcriptional regulator CynR |
| Reads supporting (aligned to +/- strand): ref base C (8/2); new base A (0/21); total (8/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.70e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATG > NZ_CP009273/353514‑353689 | aTTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaacaa > 2:111867/1‑90 (MQ=255) tttCCCCAAACACCCGCGATTTTAACCAACCCAATGTGAAAAAGTGCCTTCGGGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCaaaaaa < 1:197325/90‑1 (MQ=16) cGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccg > 1:895959/1‑90 (MQ=255) gCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTTTGCCAGCTATTTTTCCGCCGCAACAAAAccgc > 1:944173/1‑90 (MQ=255) aacaacCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGt > 1:744643/1‑90 (MQ=255) cccccGAGCATTTACCCACCGCTATGTGAAAAAATGCCTTGGGGGCGGCTGTCCGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTtc < 1:583185/87‑1 (MQ=255) cacccGCGTTTTTACCCACCCCCTTGTAAAAAAACCCCTCCGGGGCGGCTGTCTGCCAGTTTTTTTCCCCCCGAAAAAAAACCGCCGTtc < 2:96397/87‑1 (MQ=255) cacccGCGCTTTTCCCCACCCCCATGCAAAAAAATCCCTGCGCGGCGGCTGTCTGCCATTTTTTTTCCCCCCCCAAAAAACCCGCCGTtc < 2:895959/87‑1 (MQ=255) ccGCGATTTTACCCACCCCCTTGTAAAAAAATGCTTTCGGGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTtctct < 1:931138/90‑1 (MQ=255) gcATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCGGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCag > 1:849370/1‑90 (MQ=255) aCGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 2:215534/1‑90 (MQ=255) aCGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 1:200227/1‑90 (MQ=255) aCGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 1:225402/1‑90 (MQ=255) aCGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 1:898170/1‑90 (MQ=255) cATGTGAAAAAATGCCTCCGGGGCGGTTGTCTGCCAGTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAag < 1:875622/90‑1 (MQ=255) aaaaaTCCCTCCGCGGCGCCTTCCCGCCGGTTTTTTTCCCGCCAAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATa < 2:917836/90‑1 (MQ=255) aaaaaGCCCTCCGGGGGGGCTGCCGGCCATTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATa < 2:157461/90‑1 (MQ=255) ctccgcgGCGGTTGCCGGCCATTTTTTTTCCCGCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAg < 2:197957/87‑1 (MQ=255) ccggggcggtTGTCGGCCATTTTTTTTCCCGCCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 2:250244/86‑1 (MQ=255) ccggggcggcTGTCGGCCAGTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 1:180478/86‑1 (MQ=255) ccgcgGCGGCTGTCGCCCACTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 1:64483/89‑1 (MQ=255) cggcggTTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGt < 1:236005/90‑1 (MQ=255) ggggcTGTCTGCCAGTTTTTTTCCCGCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCa < 1:405135/88‑1 (MQ=255) gggcTTTCTCCCATTTTTTTTCCCCCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAt < 1:508737/89‑1 (MQ=255) tttttcCCGCCCAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 2:782136/89‑1 (MQ=255) tttttcCCGCCAAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 2:346769/89‑1 (MQ=255) tttttcCCCCCGCCAAAACCCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 1:625551/89‑1 (MQ=255) tttttcCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 1:404086/89‑1 (MQ=255) tttttcCCCCCACAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 1:28563/89‑1 (MQ=255) tttcCCGCCGCAACAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctg < 1:753037/90‑1 (MQ=255) ccccgcccAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctgct < 2:518714/88‑1 (MQ=255) gccgaAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTa < 1:464897/90‑1 (MQ=255) cccgcAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTa < 1:486857/89‑1 (MQ=255) acaaAACCGCCGTTCTCTCCAGGCGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAtg < 1:692371/90‑1 (MQ=255) | ATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATG > NZ_CP009273/353514‑353689 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |