| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 546,771 | 0 | A | G | 52.9% | 7.0 / 18.6 | 17 | T293A (ACC→GCC) | ybcF | carbamate kinase |
| Reads supporting (aligned to +/- strand): ref base A (2/6); new base G (9/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGT‑AGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACC > NZ_CP009273/546690‑546850 | ggTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTAtt < 2:798326/90‑1 (MQ=255) aTGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGc < 1:491410/90‑1 (MQ=255) cAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTa > 1:377601/1‑90 (MQ=255) gAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAACGGGGGCCCGTATTTTGCTGTAGt > 1:723049/1‑90 (MQ=255) aaGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGGAGCGGGGGCCTGTATTTTGCTGTAGtt > 2:491410/1‑89 (MQ=255) gTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGGCCTGTATTTCGCTGTAGTCGG‑Agg > 2:197458/1‑90 (MQ=255) gTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGAcc > 1:10789/1‑68 (MQ=255) gTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGAcc < 2:10789/68‑1 (MQ=255) aCCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGT‑AGGCATTAGa < 1:725692/90‑1 (MQ=255) gcgTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGGCCTGTATTTTGCTGTAGTCGG‑AGGCATTTGACAtt > 2:383946/1‑90 (MQ=255) gTTATCGCGAATTGAAGAGACGCTGGCGGGCGGAGCGGGGGCCCGTATTTTGCTGTAGTCGT‑AGGCATTAGACATTTGTGCCTGATGGGa > 2:927832/1‑90 (MQ=255) ttATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGGCCTGTATTTTGCTGTAGTCGT‑AGGCATTAGACATTTGTGCCTGATGCGAc > 2:192530/1‑90 (MQ=255) ttATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGGCCTGTATTTTGCTGTAGGCGG‑AGGGATTAGACATTTTTGCCCGATGCGAc > 2:781200/1‑90 (MQ=255) ttATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGGACCGTGTTTGGCTGTTGGCGTGAGGC‑TTAAACATTTGTTGCTGATGCGag > 2:673547/1‑89 (MQ=255) aCGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGT‑AGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCa < 1:944300/90‑1 (MQ=255) gCTGGCGGGCGAAGCGGGGGCCTGTATTTTGCTGTAGTCGT‑AGGCATTAGACATTTTTGCCCGATGCGGCGCTTGACGCGGCTTATCAgg > 2:933926/1‑90 (MQ=255) cgAAGCGGGGACCTGTATTTCGCTGTAGTCGT‑AGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAcc < 1:299569/90‑1 (MQ=255) | GGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGT‑AGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACC > NZ_CP009273/546690‑546850 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |