| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,414 | 0 | T | C | 58.3% | 5.7 / 10.7 | 12 | K99E (AAA→GAA) | ybhS | ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base T (4/1); new base C (0/7); total (4/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGG‑AATAACCACCAGACCGCGAATTTT‑CCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTG > NZ_CP009273/822347‑822495 | ggggtgGCGTGGGCGCCCCCCTTCTTTCCCGAAAAACCCCCCGG‑AATACCCCCCAGCCCGCGATTTTC‑CCCCGCCTGCATTTTGGCGATc < 2:313697/88‑1 (MQ=255)gcggtgGCGTGGGCGCGCTCCTTCTGTCCCGCAAAACCCCCGGA‑AATACCCCCCAGCCCGCGATTTTC‑CCCCGCCTGCATTTTGGCGATc < 2:510657/90‑1 (MQ=255)gcggtgGCGTGGGCCCGCCCCTTCTTTCCCGCAAAACCCCCCGA‑AATACCCCCCAGCCCGCGATTTTC‑CCCCGCCTGCATTTTGGCGATc < 2:733691/90‑1 (MQ=255)gcggggGCGTGGGCGCGCTCCTTCTTTCCCGAAAAACCCCCCGA‑AATACCCCCCAGCCCGCGATTTTC‑CCCCGCCTGCATTTTGGCGATc < 2:64417/90‑1 (MQ=255) cgcTCCATCTGTCCCGCAAAACCCCCCGA‑AATACCCCCCAGACCGCGAATTTC‑CCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTa < 1:22032/90‑1 (MQ=255) aTCTGTTCCGCAAAATCCACCGG‑AATAACCACCAGACCGCGAATTTT‑CCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTg > 1:595264/1‑90 (MQ=255) gTTCCGCAAAATCCACCGG‑AATAACCACCAGACCGCGAATTTT‑CCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGAtgg > 1:535131/1‑90 (MQ=255) cccGAAAAACCCACCGA‑AAAAACCCCCAGCCCGCAATTTTC‑CCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGAtggtg < 2:535131/89‑1 (MQ=255) aaaaCCCCCGGAAATACCCCCCAGCCCGCAATTTTC‑CCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTc < 2:373628/90‑1 (MQ=255) ccccGG‑AAAAACCCCCCGCCCCGAAATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGt < 2:279214/88‑1 (MQ=255) gACCGCGAATTTT‑CCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATgg > 1:953737/1‑90 (MQ=255) gcgAATTTT‑CCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGtgtg > 1:774179/1‑90 (MQ=255) | GCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGG‑AATAACCACCAGACCGCGAATTTT‑CCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTG > NZ_CP009273/822347‑822495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |