Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273108,7890TC51.3% 29.4 / 42.3 37S100G (AGC→GGC) zapDcell division protein ZapD
Reads supporting (aligned to +/- strand):  ref base T (11/7);  new base C (0/19);  total (11/26)
Fisher's exact test for biased strand distribution p-value = 3.72e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e-03
Rejected as consensus: Frequency below/above cutoff threshold.

GCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTGGAGTTTACGTTGC  >  NZ_CP009273/108700‑108877
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gccTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCt                                                                                          >  2:430839/1‑90 (MQ=255)
      gATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGGGAAAATTAATCCGCCCCccgc                                                                                    <  2:408098/90‑1 (MQ=255)
              gTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTa                                                                            >  1:816200/1‑90 (MQ=255)
                   tGACGCCCCAGAGCAATCAAACGATCTTCACGAAGAAATTGCCCGATACGCGGCGCGAAAATTAATACGCCCCCCGCCGCTTTTAActgc                                                                       <  1:698093/90‑1 (MQ=255)
                        cACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAAt                                                                  >  1:500752/1‑90 (MQ=255)
                        cACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAAt                                                                  >  2:422758/1‑90 (MQ=255)
                            agagCAATCAAACGATCTTCACGCAAAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAAt                                                              <  1:364574/90‑1 (MQ=255)
                            agagCAATCAAACGATCTCCCCGCAAAAATTGCCCGATACGCGGCGCGGAAATTAATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAAt                                                              <  2:171232/90‑1 (MQ=255)
                            agagCAATCAAACGATCTCCACGAAAAAATTCCCCGATACGCGGCGCGGAAATTAATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAAt                                                              <  1:742064/90‑1 (MQ=255)
                            agagCAATAAAACGATCTTCCCGCAAAATTTGCCCGATACGGGGCGCGGAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAAt                                                              <  2:791564/90‑1 (MQ=255)
                            agagAAATCAAACGATCTTCACGCAAAAATTGCCCGATCCGCGGCGCGAAAATTAATACCCCCCCCGCCGCTTTTAACTGCTGAATTAAt                                                              <  1:846111/90‑1 (MQ=255)
                               gCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCt                                                           >  1:301127/1‑90 (MQ=255)
                                 aaTCAACCGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTc                                                         <  1:18882/90‑1 (MQ=255)
                                 aaTCAACCGATCTCCACGAAAAATTTGCCCGATCCGCGGCGCGGAAATTAATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTc                                                         <  1:806567/90‑1 (MQ=255)
                                 aaTCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTc                                                         <  1:261333/90‑1 (MQ=255)
                                 aaTCAAACGACCTTCCCGCAAAATTTCCCCGATCCGCGGCGGGAAAATTAATCCGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTc                                                         <  2:593182/90‑1 (MQ=255)
                                 aaTAAAACGCTCTTCCCGCAAAAATTCCCCGATACGCGGCGGGGAAATTAATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTc                                                         <  2:258621/90‑1 (MQ=255)
                                 aaTAAAACGATCTTCCCGCAAAAATTCCCCGATCCGCGGCGGGAAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTc                                                         <  2:418319/90‑1 (MQ=255)
                                  aTCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccgc                                                                                    >  2:277617/1‑62 (MQ=255)
                                  aTCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccgc                                                                                    <  1:277617/62‑1 (MQ=255)
                                     aaaCGATCTTCACGCAGAAATTGCCCGATCCGCGGCGCGGAAATTAATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATa                                                     <  2:71643/90‑1 (MQ=255)
                                      aaCGATCTTCCCGCAGAAATTGCCCGATCCGCGGCGCGGAAATTAATACGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATAc                                                    <  1:659420/90‑1 (MQ=255)
                                      aaCGATCTCCACGAAAAAATTCCCCGATACGCGGCGCGAAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATAc                                                    <  2:301127/90‑1 (MQ=255)
                                      aaCAATCTCCACGCAAAAATTCCCCGATCCGCGGCGCGAAAATTAATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATAc                                                    <  2:500752/90‑1 (MQ=255)
                                           tctcCACGCAGAAATTCCCCGATCCGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGctc                                               <  1:212354/90‑1 (MQ=255)
                                           tCTTCACGCAAAAATTCCCCGATCCGCGGCGCGAAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGctc                                               <  2:404253/90‑1 (MQ=255)
                                            cTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGctct                                              <  1:396566/90‑1 (MQ=255)
                                                ccGCAAAAATTCCCCGATACGCGGCGCGGAAATTAATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTc                                          <  1:559511/89‑1 (MQ=255)
                                                aCGAAGAAATTCCCCGATACGCGGCGCGAAAATTAATACGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTc                                          <  1:440121/90‑1 (MQ=255)
                                                     gAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGc                                     >  2:535737/1‑90 (MQ=255)
                                                          tccccGATCGGCGGGGGGAAAATAAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGc                                <  2:841876/88‑1 (MQ=255)
                                                               gATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGcc                           >  2:847628/1‑90 (MQ=255)
                                                                    ggggcgggaAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATcc                      <  1:670195/88‑1 (MQ=255)
                                                                     cggcgcggAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCa                     >  2:324568/1‑90 (MQ=255)
                                                                                      cGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTcc                                         >  1:74559/1‑53 (MQ=255)
                                                                                      cGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTcc                                         <  2:74559/53‑1 (MQ=255)
                                                                                      cGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTGGAGTTTACGtt    >  2:785100/1‑90 (MQ=255)
                                                                                        cTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTGGAGTTTACGTtgc  >  1:667445/1‑90 (MQ=255)
                                                                                         |                                                                                        
GCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTGGAGTTTACGTTGC  >  NZ_CP009273/108700‑108877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: