| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,731 | 0 | T | A | 54.5% | 6.6 / 13.8 | 11 | L167I (TTA→ATA) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base A (6/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCT‑CCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152646‑1152816 | gCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATaat > 1:315596/1‑88 (MQ=255) tGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATgg > 1:167947/1‑90 (MQ=255) ggATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCT‑CCTTTTCCgg < 1:456578/90‑1 (MQ=255) cGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCT‑CCTTTTCCGGCATtgt < 1:777561/90‑1 (MQ=255) gCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCT‑CCTTTTCCGGCATTGTGACCt < 1:937178/90‑1 (MQ=255) actacACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTGGGTCTCGGGTTTTAAATTT‑CCTTTTTCCGCCTTTTGAGCCTccc > 1:352388/1‑89 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGGAAATATTTGGTCTCTGGTTTTTAATCTCCCTTTTCTGGGA‑TGTGACCCTCCCTAATTCgg > 2:679660/1‑90 (MQ=255) ttACAGAGGACAGAGAAACGGCGGGGAAAATAATTGGTCTCGGGTTTTTTAATT‑CCCTTTTCAGCAATGGGACCTTCCGTAATGCggggg > 2:177452/1‑87 (MQ=255) ggggAAAATAATTGATTTCGGGTTTTACATCT‑CCTTTTTCCGCATTGTGGACTTCCCTAAAGCGGGGGAAAAGCGCGGTGCTGcgcggtt > 2:497870/1‑87 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCT‑CCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:686566/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCT‑CCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:352388/90‑1 (MQ=255) | GCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCT‑CCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152646‑1152816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |