Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,812,6420AC52.0% 19.6 / 30.6 25V39G (GTG→GGG) celF6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base A (8/4);  new base C (0/13);  total (8/17)
Fisher's exact test for biased strand distribution p-value = 4.58e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-04
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCA  >  NZ_CP009273/1812567‑1812725
                                                                           |                                                                                   
aCGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCg                                                                       >  1:322586/1‑90 (MQ=255)
         ttATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGc                                                              <  1:370201/90‑1 (MQ=255)
           atcaatcaTCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCaa                                                            >  1:949362/1‑90 (MQ=255)
              aatcaTCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTc                                                         <  1:922205/90‑1 (MQ=255)
                    ccGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCCCCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATa                                                   <  1:843179/90‑1 (MQ=255)
                     cGTGGGCAGAAATCGAAAAAAATATCCATTTCCGGTTTCCCCCCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATaa                                                  <  2:707110/90‑1 (MQ=255)
                       ttGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACg                                                >  1:498407/1‑90 (MQ=255)
                        tGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTCCCACCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGc                                               <  2:453334/90‑1 (MQ=255)
                         ggCAGATATCAAAAAAAATATCAAGTTCCGGTTTACCCCCTTCGACACCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCt                                              <  2:764497/90‑1 (MQ=255)
                                     aaaTAATACCAGGTTCCGGTTTACCCCCTCCCCCATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCtt                                  <  2:580905/90‑1 (MQ=255)
                                         aataTCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAg                              >  1:673145/1‑90 (MQ=255)
                                           tatCCAGTTTCGTTTTACCACCTTCGACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTa                            <  2:446605/90‑1 (MQ=255)
                                           tatCCAGTTTCGGTTTACCCCCTCCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTa                            <  1:573326/90‑1 (MQ=255)
                                                aGTTTCGTTTTCCCACCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTcc                       <  2:259392/90‑1 (MQ=255)
                                                aGTTTCGGTTTCCCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTcc                       <  2:384966/90‑1 (MQ=255)
                                                aGTTTCGGTTTCCCACCTTCGACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTcc                       <  2:513134/90‑1 (MQ=255)
                                                aGTGTCGGTTTACCACCTTCAACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTcc                       <  2:515107/90‑1 (MQ=255)
                                                 gTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCg                      >  2:322807/1‑90 (MQ=255)
                                                 gTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCg                      >  1:201579/1‑90 (MQ=255)
                                                  tttCGGTTTACCCCCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCgg                     <  1:788860/90‑1 (MQ=255)
                                                           accaccTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAgct            >  1:233888/1‑90 (MQ=255)
                                                             caccTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAgctgc          <  1:623325/90‑1 (MQ=255)
                                                                cTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTcc       >  2:451153/1‑90 (MQ=255)
                                                                  tCGACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCg     <  2:335434/90‑1 (MQ=255)
                                                                     acacCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAACCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGcca  <  2:322586/90‑1 (MQ=255)
                                                                           |                                                                                   
ACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCA  >  NZ_CP009273/1812567‑1812725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑