| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,096 | 0 | T | G | 52.6% | 16.3 / 25.0 | 19 | V415G (GTG→GGG) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (6/3); new base G (0/10); total (6/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.10e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCA‑GCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGG > NZ_CP009273/3214013‑3214185 | aGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCA‑GCGAGATGTTCCGCCAGCGCGTACTGGTGGCCgg < 1:630918/90‑1 (MQ=255) gTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCA‑GCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCt > 2:352159/1‑90 (MQ=255) gTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCA‑GCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCt > 1:826740/1‑90 (MQ=255) tGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCA‑GCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATa < 2:375840/90‑1 (MQ=255) gttgATAACGAAATCGGCTATAACTTTGCCA‑GCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATc < 1:141381/90‑1 (MQ=255) aaaTCGGCTATAACTTTGCCA‑GCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAc > 2:765037/1‑90 (MQ=255) agcGGCTAAATCTTCGCCA‑GAGACATGTCCCGCCGGCGCGTACGGGGGCCGGGAACCCTAAAAAACCCCAAAACGACCCGCATTGAACCg < 2:764720/88‑1 (MQ=255) aCTTTGGCAGGCGAGTTTTCCCGCCGGCGCGTACGGGGGGCGGGACCGCTAAAAAACCCAAAAACGACCCGCATTGAACCGCcactgaca < 1:525925/90‑1 (MQ=255) cTTTGCGG‑GCGAGTTGTCCCGCCGGCGCGTCCGGGGGGCCGGAACGCTAAATAACGCAAAAACGACCCGCATGGACCCGCcactgacact < 2:377661/90‑1 (MQ=255) tttGCCG‑GCGAGTTGTCCCGCCGGCGCGTCCTGGGGGCCGGACCGCTAAATAACCCCAAAACGACCCGCATTGAACCGCcactgacactg < 1:489307/90‑1 (MQ=255) tttGCCA‑GCGAGTTGTCCCGCCAGCGCGTCCTGGGGGCCGGACCGCTCAAAACCGCAAAAACGACCCGCATTAAACCGCcactgacactg < 1:235644/90‑1 (MQ=255) tttGCCA‑GAGAAATTTCCCGCCAGCGCGTACTGGGGGCCGAAACGCTCAATACCCCCAAAACGATCCGCATTGAACCGCcactgacactg < 1:283259/90‑1 (MQ=255) tttCCCA‑GCGAGATGTCCCGCCAGCGCTTACGGGGGGCCGGAACGTTCAAAACCGCCAAAACGACCCGCATTAAACCGCcactgacactg < 1:95605/90‑1 (MQ=255) cggggagtTGTCCCGCCGGCGCGTCGGGGGGGCGGAAACGCCAAAAAACCCAAAAACGACCCGTATTAAACCGCCACTGACACTGACCAt < 2:316761/86‑1 (MQ=255) gCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTg > 2:532784/1‑90 (MQ=255) ggagaTGTCCCGCCAGCGCGTGGTGGGGGCCGGAACGCTAAAAAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGa < 2:192510/89‑1 (MQ=255) gagaTGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGaa > 1:938782/1‑90 (MQ=255) gttGTCCCGCCGGCGCGTCCGGGGGGCCGGAACGCTAAAAAACGAAAAAACGCCCCGCATTGAACCGCCACTGACACTGACCATTGAACa < 2:368399/88‑1 (MQ=255) aGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTgg > 1:131577/1‑90 (MQ=255) tggCCGAACCGTTCAAAAACACAAAAACGCTCCGCATTAAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgcgg < 1:347919/90‑1 (MQ=255) | AGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCA‑GCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGG > NZ_CP009273/3214013‑3214185 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |