| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,726,640 | 0 | T | A | 47.6% | 18.9 / 10.3 | 21 | N360K (AAT→AAA) | xylG | D‑xylose ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/11); new base A (10/0); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.84e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAA > NZ_CP009273/3726554‑3726721 | cAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATAtt < 2:516860/90‑1 (MQ=255)cAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAAAAtt > 1:735094/1‑90 (MQ=255) aaGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTAccc < 2:735094/90‑1 (MQ=255) gCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAAAATTACCCTc > 1:258869/1‑90 (MQ=255) gCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGGTGGGAAAAAAATTAACCTc > 2:918063/1‑90 (MQ=255) cATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGGAAAAAAATTAACCTcgc > 2:430289/1‑90 (MQ=255) gCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCa < 1:657311/90‑1 (MQ=255) cccAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCAc < 2:860553/90‑1 (MQ=255) ccAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACt < 2:573405/90‑1 (MQ=255) gATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTGAAAAAATTAACCCCCCCCCCCTCCATaa > 2:924129/1‑90 (MQ=255) ttGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAAAATTACCCCCCCCCCACTCAATAAAt > 1:882432/1‑90 (MQ=255) gATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGGAAAAAAATTACCCCCGCCGCACTCAAAAAAatttc > 1:59942/1‑88 (MQ=255) aTGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGGAAAAAAATTACCCCCCCCGCACTCAATAAATTTAcc > 1:297732/1‑90 (MQ=255) ggTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGGAAAAAAATTACCCCCCCCCCACCCAAAAAAATTTCCgg > 1:519483/1‑90 (MQ=255) gTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGt < 1:657587/90‑1 (MQ=255) cAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGcta < 1:435064/90‑3 (MQ=255) gcgACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAAAATTAACCCCCCCCCCCTCAATAAAATTTCCGGGGGGATTTGCCCGCTtgatg > 2:372675/1‑90 (MQ=255) cGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGAcgc < 2:676490/90‑1 (MQ=255) ccGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCaa < 2:523581/90‑1 (MQ=255) aaTGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATg < 2:292832/90‑1 (MQ=255) ggTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGaa < 1:378026/90‑1 (MQ=255) | CAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAA > NZ_CP009273/3726554‑3726721 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |