| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,781,385 | 0 | T | G | 54.5% | 16.4 / 27.3 | 22 | G396G (GGT→GGG) | envC | murein hydrolase activator EnvC |
| Reads supporting (aligned to +/- strand): ref base T (3/7); new base G (12/0); total (15/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGATAAGTTTTGTTTCCATTT > NZ_CP009273/3781298‑3781472 | cTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCg > 1:141647/1‑90 (MQ=255) agagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCAc < 1:276818/90‑1 (MQ=255) gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCAct > 1:182357/1‑90 (MQ=255) gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCAct > 1:392537/1‑90 (MQ=255) gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGGTAGGGGCGGCCTTCCct > 1:605088/1‑90 (MQ=255) cTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTc < 1:630103/90‑1 (MQ=255) ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTcgccgc > 1:79918/1‑90 (MQ=255) gcgGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc < 1:103869/90‑1 (MQ=255) gcgGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc < 2:909038/90‑1 (MQ=255) gcgGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc < 2:354483/90‑1 (MQ=255) aTTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCg < 1:874988/90‑1 (MQ=255) ttGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCCCTCTTTTTTGAAAATTGCCCCCCGGGGCAGGCGGGCAATCCCCAGCCgg > 2:953650/1‑89 (MQ=255) aCTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCGTTACTCTATTTTGGAAATGGCCGGCAAGGGCAGGGGGGCAATACAGAGCCgggggt > 1:944273/1‑88 (MQ=255) gtggGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGGCAGGCGGTCAATCCACAGCCGGGGGTgggg > 1:824456/1‑89 (MQ=255) gcagcaGTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGGCAATCCACAGCCGTGGGTGGGGAGAt > 2:350658/1‑90 (MQ=255) gcagcaGTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTCGCCCCCAGGGGCAGGGGGGCAATCCACAGCCGTGGGTGGGGAGAt > 1:664686/1‑90 (MQ=255) gcagTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGGCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGATAAg > 1:158875/1‑90 (MQ=255) agTGGCGGTCAGGGTCGGCCTTCACTCTATTTTGAAATTCGCCGCCAGGGGCAGGCGGGCAATCCCCAGCCGTGGGTGGGGAGATAAggt > 2:861879/1‑88 (MQ=255) agTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTCGCCGCCCGGGGCAGGGGGGCAAACCCCAGCCGTGGTTGGGGAGATAAGtt > 2:633426/1‑90 (MQ=255) ggTCAGGGTCGGCCTTCACTCTATTTCGAAAATCGCCGCCAGGGGCAGGCGGGCAATCCACAGCCGTGGGTGGGAAGATAAGTTTTGttt > 1:615537/1‑90 (MQ=255) ggTCAGGGGCGGCCCTCACTCTTTTTTGAAATTCGCCGCCAGGGGCAGGGGGGCAATCCCCAGCCGGGGGTGGGGAGATAAGATTTGttt > 2:592020/1‑90 (MQ=255) ggTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGATAAGTTTTGTTTCCAttt < 2:182357/90‑1 (MQ=255) | CTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGATAAGTTTTGTTTCCATTT > NZ_CP009273/3781298‑3781472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |