| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,464,532 | 0 | T | G | 53.3% | 56.3 / 60.9 | 62 | *198G (TGA→GGA) | bdcR | TetR/AcrR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (11/17); new base G (32/0); total (45/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.55e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.48e-10 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAA > NZ_CP009273/4464443‑4464617 | cTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGt < 1:688387/90‑1 (MQ=255) aaGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGc < 2:581043/90‑1 (MQ=255) aGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCg < 2:826827/90‑1 (MQ=255) aGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCg < 2:105830/90‑1 (MQ=255) ggggCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGGGATAAAAAATATTTTTGAGGAGTGGTGGGGGGCTTGAcc > 1:433390/1‑90 (MQ=255) gggCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATTTTCTTGAGGGGAGATGCGGGGCTTGAccc > 1:739710/1‑90 (MQ=255) gCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTAGGAGGGATGCGGGGCTTGACCCGa > 2:367895/1‑90 (MQ=255) aCTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGGTGCGGGCCTTGACCCGaaa > 1:114716/1‑90 (MQ=255) ctctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAAAATTTTTTAGGAGGGAGGCGGGGCTTTGCCCGGAAg > 2:940460/1‑90 (MQ=255) ctctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTTTTGGGGGGGGGTGCGGGGCTTGGGCCGGAAg > 2:866439/1‑90 (MQ=255) tctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAgg > 1:5474/1‑90 (MQ=255) ataGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTGGGGGTGATGCGGGGCTTGACCCGAAAGGCgg > 2:673041/1‑90 (MQ=255) taGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGGGGGATGCGGGGCCTGACCCGAAAAGCggg > 2:112273/1‑90 (MQ=255) caacTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTTAGGGGGGATGCGGGGCTTGACCCGAAAGGCGGGGAAga > 2:506535/1‑89 (MQ=255) caacTCTGTGCAACAGCTGCAATGGCGGGGGGAACGATAAAAA‑TATTCTTTGGGAGAGATGCGGGCCCTGACCCGAAAGGGGGGGAAacg > 2:878104/1‑90 (MQ=255) caacTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAgc > 1:932929/1‑90 (MQ=255) caacTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGAAAgc > 1:338899/1‑90 (MQ=255) caacTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGAAAgc > 1:330633/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAGTGGCGGGGGGAGCGATAAAAACTATTTTTGAGGGGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAgcgc > 1:346857/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAATATTCTTTAGGGGGGATGGGGGCCTTTGCCCGAAAGGGGGGAAAgcgc > 1:478634/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTTTTTTGGGGGGATGGGGGCCTTGACCCGAAAGGGGGGGAAacgc > 2:596280/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTTTTGAGGGGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAgcgc > 1:733172/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTTTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAacgc > 2:337584/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAacgc > 2:821257/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAacgc > 2:386825/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGTGGAGGGATGCGGGGCCTGGCCCGAAAGGCGGGAAAacgc > 2:498765/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGAGGGATGCGGGGCTTGACCCGAAAGGCGGGGAAacgc > 2:826993/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAacgc > 2:241455/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTTAGGGGGGATGCGGGCCTTGACCCGAAAAGCGGGGAAgcgc > 1:885997/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGAAAgcgc > 1:787916/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTCTTTAGGAGGGATGCGGGGCTTGACCCCAAAGGCGGGGAAacgc > 2:238521/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGAAAAAAACTATTTTTTAGGAGGGATGCGGGCCTTGACCCGAAAAGCGGGaaaaagc > 2:640176/1‑86 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGGAAacgc > 1:105964/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAgcgc > 1:662953/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAgcgc > 1:171789/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGAAAgcgc > 1:374515/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGGAAgcgc > 1:785691/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAAGCGGGGAAacgc > 2:191672/1‑90 (MQ=255) ctctGTGCAACAGCTGCAATGGGGGGGGAAGCGAAAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGcc < 2:895041/90‑1 (MQ=255) ctctGTGCAACAGCTGAAAGGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGcc < 2:76630/90‑1 (MQ=255) tctGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAAAATTTTTTAGGGGGGATGCGGGGCTTGACCCGAAAGGCGGGGAAAAGCCt > 2:901629/1‑90 (MQ=255) tctGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTTTTTAGGAGGGATGCGGGCCCTGAGCCGGAAGGCGGGGAAACGccc > 2:584614/1‑89 (MQ=255) gtgCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAATATTTTTGAGGGGGGATGCGGGCCTTGACCCGAAAGACGGGAAAACGCCTGcc > 2:156115/1‑90 (MQ=255) gtgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGcc > 1:59391/1‑90 (MQ=255) cAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCCAAAGGCGGGAAAACGCCCGCCGAt > 1:951100/1‑90 (MQ=255) aaCAGGTGCAATGGCGGGGGAAGCGATAAAAACTTTTCTTGGGGAGTGAGGGGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATa < 2:721886/90‑1 (MQ=255) aaCAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGACGGGAAAGCGCCTGCCGATa < 1:156115/90‑1 (MQ=255) aGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGt < 2:338899/90‑1 (MQ=255) gCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGtt < 1:7609/90‑1 (MQ=255) gCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGtt < 2:82231/90‑1 (MQ=255) tGCAATGGCGGGGGGAGGGGTAAAAACTATTTTTGAGGAGGGGTGCGGGGCCTGACCCGAAAGGCGGGGGAGCGCCCGCCGATAAggttt > 2:672073/1‑86 (MQ=255) tGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGGGGGAAAAGGCCTGCCGATAAGTtgg > 2:516367/1‑89 (MQ=255) tGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGCCCTGACCCGAAAGGCGGGAAAGCGCCTGCCGCTAAGTtgt > 2:918160/1‑90 (MQ=255) aTGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATa < 1:669892/90‑1 (MQ=255) gCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTc > 2:535648/1‑90 (MQ=255) gATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCAc < 1:579092/90‑1 (MQ=255) gATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCAc < 1:901629/90‑1 (MQ=255) aaCTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTa < 1:830253/90‑1 (MQ=255) aaCTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTa < 1:506535/90‑1 (MQ=255) tgttCTTGGGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAgg < 2:341294/88‑1 (MQ=255) tGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCg > 2:791996/1‑90 (MQ=255) gaggagTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCgg > 2:730930/1‑90 (MQ=255) ggagTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGaa < 2:662953/90‑1 (MQ=255) | CTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAA > NZ_CP009273/4464443‑4464617 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |