Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,594,161	0	C	T	57.3%	12.9 / 85.9	75	S62F (TCT→TTT)	bglJ	DNA‑binding transcriptional activator BglJ
Reads supporting (aligned to +/- strand):  ref base C (6/26);  new base T (43/0);  total (49/26)
Fisher's exact test for biased strand distribution p-value = 8.96e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.88e-10
Rejected as consensus: Frequency below/above cutoff threshold.

CCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTT  >  NZ_CP009273/4594078‑4594245                                                                                    |                                                                                     cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                <  1:921580/90‑1 (MQ=255) cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                <  1:739591/90‑1 (MQ=255) cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                <  1:34591/90‑1 (MQ=255) cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                <  2:1191612/90‑1 (MQ=255) cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTCTGCGGCGGTCATtttttctttttc                                                                                <  2:941715/90‑1 (MQ=255)   cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTg                                                                              <  2:1139176/90‑1 (MQ=255)    ttATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGc                                                                             >  1:811702/1‑90 (MQ=255)     tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGcc                                                                            <  2:1063582/90‑1 (MQ=255)          gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTgag                                                                       >  1:806071/1‑90 (MQ=255)          gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATgag                                                                       >  2:734432/1‑90 (MQ=255)           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttttc                                                                                >  1:647105/1‑80 (MQ=255)           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                <  2:647105/80‑1 (MQ=255)           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTgaga                                                                      >  2:334959/1‑90 (MQ=255)            tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCGTGAGaa                                                                     <  1:63548/90‑1 (MQ=255)            tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGaa                                                                     <  1:514779/90‑1 (MQ=255)             ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAg                                                                    >  2:750673/1‑90 (MQ=255)             ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTCGCCCTGAGAAg                                                                    >  2:270874/1‑90 (MQ=255)             ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCCTGAGAAg                                                                    >  1:426749/1‑90 (MQ=255)               aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAggg                                                                  >  1:36779/1‑88 (MQ=255)               aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTg                                                                  <  1:31965/90‑1 (MQ=255)                 aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAg                                                                <  1:95475/90‑1 (MQ=255)                    tatcccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCCTTTTTTTTTTTTTTTCCCTGAGAAGTGAgggc                                                             >  1:1057782/5‑90 (MQ=255)                    tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGGgcgc                                                             >  1:161814/1‑90 (MQ=255)                    tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGGAGGGAgggc                                                             >  1:1056302/1‑90 (MQ=255)                    tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                             <  2:882206/90‑1 (MQ=255)                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGGGCa                                                            >  1:112182/1‑90 (MQ=255)                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGAGAGGGCa                                                            >  2:201784/1‑90 (MQ=255)                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCa                                                            >  1:484787/1‑90 (MQ=255)                      taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGGag                                                           >  1:958972/1‑90 (MQ=255)                       aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                          <  2:417651/90‑1 (MQ=255)                        ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                         <  1:595856/90‑1 (MQ=255)                        ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                         <  1:388178/90‑1 (MQ=255)                         cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                        <  1:589573/90‑1 (MQ=255)                         cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                        <  1:859981/90‑1 (MQ=255)                         cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                        <  2:672390/90‑1 (MQ=255)                          aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCagagag                                                       >  1:1206940/1‑90 (MQ=255)                          aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCagggag                                                       >  2:154/1‑90 (MQ=255)                          aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGATCATTTTTTTTTTTTTTTCCATGAGAAGGGGGCGCagagag                                                       >  2:956659/1‑90 (MQ=255)                           gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAgg                                                      >  1:166989/1‑90 (MQ=255)                           gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGGAGAGAgg                                                      >  1:1088627/1‑90 (MQ=255)                           gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGAGAgg                                                      >  2:1234581/1‑90 (MQ=255)                           gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGAGGGCGCAGAGAgg                                                      >  2:806371/1‑90 (MQ=255)                           gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGGGCAGAgggg                                                      >  1:539700/1‑90 (MQ=255)                           gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAgg                                                      >  2:907733/1‑90 (MQ=255)                           gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGGAGGGAGCGCAGAGAgg                                                      >  1:710891/1‑90 (MQ=255)                           gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAgg                                                      >  1:9150/1‑90 (MQ=255)                            tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCAGgggggg                                                     >  1:271073/1‑90 (MQ=255)                            tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAggg                                                     >  1:24620/1‑90 (MQ=255)                            tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCAGAGAggg                                                     >  2:623404/1‑90 (MQ=255)                            tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAggg                                                     >  2:117006/1‑90 (MQ=255)                            tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCagggaggg                                                     >  2:780978/1‑90 (MQ=255)                            tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGGGAGCGCAGAGAggg                                                     >  2:176520/1‑90 (MQ=255)                             cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCGTTTTTTTTTTTTTTTGGATGAGGAGGGCGAGCAGAGAgggg                                                    >  2:103931/1‑89 (MQ=255)                                      tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc                                           <  2:967544/90‑1 (MQ=255)                                      tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc                                           <  1:444402/90‑1 (MQ=255)                                      tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc                                           <  1:956659/90‑1 (MQ=255)                                       ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGcc                                          <  1:1025086/90‑1 (MQ=255)                                           ggATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGGGCAGAGAGGGATTATCTTGCctgac                                      >  2:1183339/1‑90 (MQ=255)                                              tGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCAGAGAGGGGTTATCTTGGctgactga                                   >  2:140571/1‑90 (MQ=255)                                              tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCAGAGAGGGGTTATCTTTCctgactga                                   >  2:966630/1‑90 (MQ=255)                                              tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTTCctgactga                                   >  2:487603/1‑90 (MQ=255)                                               gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGaa                                  >  2:173254/1‑90 (MQ=255)                                                ccATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGGGAGGGGTTATTTTGCCTGACTGAAc                                 >  1:306037/1‑90 (MQ=255)                                                ccATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAAc                                 >  2:558037/1‑90 (MQ=255)                                                       gcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGAt                          <  1:238597/90‑1 (MQ=255)                                                       gcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGAt                          >  2:238597/1‑90 (MQ=255)                                                         ggATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATTTTGCCTGACTGAACTGGCGATTa                        >  2:72679/1‑90 (MQ=255)                                                         ggATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTa                        <  2:1202262/90‑1 (MQ=255)                                                            tctcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGt                     >  2:90848/1‑90 (MQ=255)                                                              tcGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGGAGGGGGCGGGGGGGGGGGGTATCTTTTCTGGCTTAACTTGCGATTTAGttt                   >  1:593365/1‑90 (MQ=255)                                                              tcGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCGGGGAGGGGTTATCTTGCCTGACTGAACTTGCGATTaaagtt                   >  2:1221865/1‑86 (MQ=255)                                                                  ttGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCcgc               <  2:866405/90‑1 (MQ=255)                                                                   tGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCcgcg              <  2:710891/90‑1 (MQ=255)                                                                    gcggcgGGCATTTTTTCTTTTTTTGCCATGAGAAGTGGGCGCAGAGAGGGATTATCTTGCCTGGCTGAACTGGCGGTTAAGTTTCcccgg             >  2:1034278/1‑89 (MQ=255)                                                                       gcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcc          <  2:913894/90‑1 (MQ=255)                                                                         gggcATTTTTTTTTTTTTTGCCATGAGAAGGGGGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGGTTTAGTTTTCGCGTACCCg        >  1:480063/4‑90 (MQ=255)                                                                         ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCg        >  1:426474/1‑90 (MQ=255)                                                                         ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCg        <  2:761762/90‑1 (MQ=255)                                                                         ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCg        <  1:1048537/90‑1 (MQ=255)                                                                         ggTCATTTTTTCTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCg        >  2:190936/1‑90 (MQ=255)                                                                             aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGt    <  1:1163189/90‑1 (MQ=255)                                                                             aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGt    <  2:870257/90‑1 (MQ=255)                                                                               tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGttt  <  2:426474/90‑1 (MQ=255)                                                                                    |                                                                                     CCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTT  >  NZ_CP009273/4594078‑4594245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑