| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 595,035 | 0 | T | G | 52.6% | 9.1 / 22.5 | 19 | V289G (GTG→GGG) | cusA | Cu(+)/Ag(+) efflux RND transporter permease subunit CusA |
| Reads supporting (aligned to +/- strand): ref base T (1/8); new base G (10/0); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.29e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGA > NZ_CP009273/594956‑595119 | gCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCgg > 2:318929/1‑89 (MQ=255) ggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGT‑CCgg > 1:218024/1‑90 (MQ=255) ggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGAtaccg > 2:772218/1‑86 (MQ=255) tGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGc < 2:1260675/90‑1 (MQ=255) ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCCGCGGT‑CCGGCaaaaaaacc > 1:125841/1‑86 (MQ=255) ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGAT‑CCGGCAAAAACGcc > 2:1023778/1‑90 (MQ=255) cccGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCg < 2:70751/90‑1 (MQ=255) cGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCgag < 1:45923/90‑1 (MQ=255) gagaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGT‑CCGGCAAAAAAGCCCCAGaa > 2:903616/1‑90 (MQ=255) gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGT‑CCGGCAAAAAAACCCCAGAAGGGAt > 1:408735/1‑90 (MQ=255) gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTACTGCGAT‑CCGGGAAAAAAGCCCCAGAAGGGAt > 2:572215/1‑90 (MQ=255) gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATTCTTCGGT‑CCCGCAAAAAAGCCCGAGAAGTgga > 2:903434/1‑88 (MQ=255) ccGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATcgc < 2:392994/90‑1 (MQ=255) gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATcgccgc < 2:718920/90‑1 (MQ=255) ttGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGGGa > 2:514200/1‑90 (MQ=255) gAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGTCCCGGCAAAAAC‑CCCCAGAAGGGGGTGCCGCCGGGGAgggc > 1:1162515/1‑88 (MQ=255) aggcgaagTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCt < 1:903616/90‑1 (MQ=255) cgggcgggGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTc < 2:408735/90‑1 (MQ=255) cgggcgggGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTc < 1:450126/90‑1 (MQ=255) ggtggtGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGa < 2:935360/90‑1 (MQ=255) | GCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGA > NZ_CP009273/594956‑595119 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |