| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,235,279 | 0 | T | G | 57.1% | 4.7 / 10.5 | 14 | G315G (GGT→GGG) | dadX | catabolic alanine racemase DadX |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (8/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGGCGTGCGCACCATGACGGTGGGGACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGTATTGGTACGCCGGTTGAGCTGTGGGGCAAGGAGATCAAAATTGATGATGTCGCCGCCGCTGCCGGAACGGTGGGCTATGAGTTGATG > NZ_CP009273/1235200‑1235366 | aCGGCGTGCGCACCATGACGGTGGGGACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGGATTGGGACGc > 2:173677/1‑90 (MQ=255) tGCGCACCATGACGGTGGGGACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGGATTGGTACGCCGGTTg > 2:1161482/1‑90 (MQ=255) gTGGGGACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGGATTGGGACGCCGGTTGAGGTGTGGGGGAAg > 1:1236508/1‑90 (MQ=255) ggggACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGGATTGGGACGCCGGGTGAGCTGTGGGGCAAGga > 2:270809/1‑90 (MQ=255) ccGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGGATTGGTACGCCGGTTTAGCTGTGGGGGAAAGAGAtta > 2:1089534/1‑88 (MQ=255) cGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGTATTGGGACGCCGGTTGAGCTGTGGGGCAAGGAGATCaa > 1:108052/1‑90 (MQ=255) gTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGGATTGGTACGCCGGTTGGGCTGTGGGGGAAGGAGATCaaa > 2:1165928/1‑90 (MQ=255) gATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGGATTGGTACGCCGGGTGAGCTGTGGGGGAAGGGGAGCAAAAATgatgat > 1:860920/1‑90 (MQ=255) gCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGGATTGGTACGCCGGTTGAGCTGTGGGGCAAGGAGATCAAAATTGATGATGTcgc > 2:222218/1‑90 (MQ=255) cGGTCGATTTAACGCCTTGCCCGCAGGCGGGTATTGGTACGCCGGTTGAGCTGTGGGGCAAGGAGATCAAAATTGATGATGTcgccgccg < 1:1161482/90‑1 (MQ=255) ccTTGCCCGCAGGCGGGTATTGGTACGCCGGTTGAGCTGTGGGGCAAGGAGATCAAAATTGATGATGTCGCCGCCGCTGCCGGAACGGTg < 1:1165928/90‑1 (MQ=255) ttGCCCGCAGGCGGGTATTGGTAAGCCGGGTGAGCTGGGGGGCAAGGAGATCAAAAATTATGATGTCGCCGCCGCTGCCGGAACgggggg > 1:1219930/1‑90 (MQ=255) cGGGTATTGGTACGCCGGTTGAGCTGTGGGGCAAGGAGATCAAAATTGATGATGTCGCCGCCGCTGCCGGAACGGTGGGCTATGAGTTGa < 2:860920/90‑1 (MQ=255) ggTATTGGTACGCCGGTTGAGCTGTGGGGCAAGGAGATCAAAATTGATGATGTCGCCGCCGCTGCCGGAACGGTGGGCTATGAGTTGAtg < 1:270809/90‑1 (MQ=255) | ACGGCGTGCGCACCATGACGGTGGGGACCGTCTCGATGGATATGCTAGCGGTCGATTTAACGCCTTGCCCGCAGGCGGGTATTGGTACGCCGGTTGAGCTGTGGGGCAAGGAGATCAAAATTGATGATGTCGCCGCCGCTGCCGGAACGGTGGGCTATGAGTTGATG > NZ_CP009273/1235200‑1235366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |