Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,380,3620CT54.2% ‑2.5 / 26.9 24intergenic (+52/‑51)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
Reads supporting (aligned to +/- strand):  ref base C (3/8);  new base T (13/0);  total (16/8)
Fisher's exact test for biased strand distribution p-value = 2.24e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-04
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAA  >  NZ_CP009273/2380278‑2380451
                                                                                    |                                                                                         
ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGGCCGAAAAAAAGACAAGGACATTTTTTTTTTCCTCa                                                                                      >  2:614446/1‑90 (MQ=255)
  tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc                                                                                    <  1:101278/90‑1 (MQ=255)
  tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc                                                                                    <  2:552132/90‑1 (MQ=255)
      ataACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTATTTTTCCTCATCTGtt                                                                                >  2:619860/1‑90 (MQ=255)
          cTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTaa                                                                            <  1:1185206/90‑1 (MQ=255)
          cTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTaa                                                                            <  1:566789/90‑1 (MQ=255)
           tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACAAGCACATTTTTTTTTTCCTCCTCTGtttttaa                                                                           >  2:796970/1‑87 (MQ=255)
           tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGAAATGCACATTTTTTTTTTTCTCATCTGtttttaa                                                                           >  1:233853/1‑87 (MQ=255)
           tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTACCGAAAAAGCCACGAGCACATTTTTTTTTTCCTTCGCTGtttttga                                                                           >  2:783485/1‑87 (MQ=255)
           tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAGGACATTTTTTTTTTCCTCCTCtttttttaa                                                                           >  1:538920/1‑87 (MQ=255)
           tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTTCTCCTCtttttttaa                                                                           >  1:32116/1‑87 (MQ=255)
           tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCCTCTGtttttaa                                                                           >  2:887280/1‑87 (MQ=255)
           tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGAAAAGGACAATTTATTTTTCCTCCTCCGtttttaa                                                                           >  1:495791/1‑87 (MQ=255)
           tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGAAAAGCACATTTTTTTTTTTCTCCTCTGTTTTTAg                                                                           >  1:112809/1‑90 (MQ=255)
                 ataCAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGAAAAGCACATTTTTTTTTTCCTCCTCTTTTTTTAAATGCGa                                                                     >  2:62642/1‑90 (MQ=255)
                    caacaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAAc                                                                  >  2:125126/1‑90 (MQ=255)
                      acaGTAAATAATAAAAATTTCTCGAGGCGGGCGCAGAAAAAATGACATGGACATTTTATTTTCCCTTATCTGTTTTTAGAAGCGaaaaaa                                                                >  2:1144405/1‑87 (MQ=255)
                           aaaTAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACAATTTTTTTTTTCTCCTCTGTTTTTAGATGGGAAAAAAGaaaa                                                           >  1:1015164/1‑89 (MQ=255)
                                      aTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagga                                                <  1:783485/90‑1 (MQ=255)
                                       tttCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTATTTTTCCTCCTCTGTTTTAAGAAGCGGAAAAAGAAAATCAACAGAGGaa                                               >  2:780912/1‑90 (MQ=255)
                                              aGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaataaa                                        <  2:112809/90‑1 (MQ=255)
                                                             aaGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCt                         >  2:52605/1‑90 (MQ=255)
                                                                      gCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAg                <  2:1152009/90‑1 (MQ=255)
                                                                               attttCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACt       <  1:836433/90‑1 (MQ=255)
                                                                                    cccTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGaa  <  1:866184/90‑1 (MQ=255)
                                                                                    |                                                                                         
GGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAA  >  NZ_CP009273/2380278‑2380451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑