| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,636,048 | 0 | T | G | 54.5% | 8.0 / 12.6 | 11 | A52A (GCA→GCC) | rodZ | cytoskeleton protein RodZ |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (6/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTACCGT‑GGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCG > NZ_CP009273/2635967‑2636137 | cagTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGGCttttt > 1:258460/1‑87 (MQ=255) agTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGTGCCTTAtct < 2:119588/90‑1 (MQ=255) gacagcgcATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTACCGT‑GGaa < 2:418730/86‑1 (MQ=255) aaGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTACCGT‑GGAAACCTTCAGGc < 2:1155781/90‑1 (MQ=255) ggATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGGCTTTTTTTTTTTCATTTCGCGGGCCCTGGGAAACCTCCGGGAAAAgc > 2:1100943/1‑89 (MQ=255) gATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGGCGTATTTTTTTTTATTTTGCGTGCCGG‑GGGAAACCTCACGCAAAATCg > 1:969011/1‑90 (MQ=255) atatatCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTACCGT‑GGAAACCTTCAGGCAAAGTCGc < 2:1043467/90‑1 (MQ=255) gcgcAGGAATGTTGAAGCAAGATCGGCGGGGGGCTTTTCTTTTTTCATTTTGCGTACCGT‑GGGAAACCTCAGGGAAAAAAGCTCGGCAAc > 1:20528/1‑90 (MQ=255) gcgcAGGAATGTTGAAGCAAGATCGGCGGGGGCCTTATCTTTTTCAATGTCGCGGACCGT‑GGGAAACTTCAGGCAAAAACGCTCCGCAAc > 2:362337/1‑90 (MQ=255) cgcAGGAATGTTGAAGCAAGATCGGCGGGGGGCTTTTTTTCTTTTATTTCGCGTACCGT‑GGAAAACTTCAAGGAAAATCGCTCGGCCACg > 2:292996/1‑90 (MQ=255) aaGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTACCGT‑GGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTaa < 2:1002754/90‑1 (MQ=255) tGCCTTATCTTCTTCAATGTCGCGTACCGT‑GGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTcgcg < 1:1126046/90‑1 (MQ=255) tGCCTTATCTTCTTCAATGTCGCGTACCGT‑GGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTcgcg < 2:681567/90‑1 (MQ=255) tGCCTTATCTTCTTCAATGTCGCGTACCGT‑GGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTcgcg < 2:773566/90‑1 (MQ=255) | CAGTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTACCGT‑GGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCG > NZ_CP009273/2635967‑2636137 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |