Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,738,4480AC55.3% 10.5 / 33.1 38V84G (GTG→GGG) trmDtRNA (guanosine(37)‑N1)‑methyltransferase TrmD
Reads supporting (aligned to +/- strand):  ref base A (9/8);  new base C (0/21);  total (9/29)
Fisher's exact test for biased strand distribution p-value = 1.49e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.16e-03
Rejected as consensus: Frequency below/above cutoff threshold.

GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCC  >  NZ_CP009273/2738359‑2738533
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gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                       >  1:1191676/1‑90 (MQ=255)
gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                       >  1:682740/1‑90 (MQ=255)
       cacCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTgc                                                                                <  1:99422/90‑1 (MQ=255)
         ccAGAATCAATTTTTGATTCGTTGCCATTTCGCTGCCGCCCGCTTGATCAAGTTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTgcgc                                                                              <  1:824564/90‑1 (MQ=255)
              aTCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCa                                                                         >  2:1220995/1‑90 (MQ=255)
                 attttttGATTCGTTGCCGTTTCGCTGACGCCCGCTTGATAAAGCTTGGGTCCGTGTGGTGAAAGATACTTCACCTTTGCGCTTTCcccc                                                                      <  1:1220995/88‑1 (MQ=255)
                 aaTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCAccc                                                                      >  1:766440/1‑90 (MQ=255)
                     tttGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCccgccg                                                                  <  2:786720/90‑1 (MQ=255)
                       tGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCcgcg                                                                <  2:393976/90‑1 (MQ=255)
                       tGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCcgcg                                                                >  2:1162825/1‑90 (MQ=255)
                            cGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCttt                                                           >  1:309665/1‑90 (MQ=255)
                             tttcccAGTTCGCTGCCGCCCCCTTAATCACGCTTGCCTCCCTGTGGTGACAGAAAAATCACCTTTGCGCCTTCCCCCGCCGCGGCtttt                                                          <  1:973951/86‑1 (MQ=255)
                                gCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTtgct                                                       <  2:309665/90‑1 (MQ=255)
                                gCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTtgct                                                       <  2:392628/90‑1 (MQ=255)
                                    gTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCAt                                                   >  1:319204/1‑90 (MQ=255)
                                     ttCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATg                                                  <  2:515021/90‑1 (MQ=255)
                                          cgACGCCCGCTTGATCAAGCTTGCGTCCTTGTGGGGACAGAAAAATCCCCTTTGCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATgg                                             <  1:901785/89‑1 (MQ=255)
                                              gCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTc                                         <  1:1135806/90‑1 (MQ=255)
                                                   cTTGATCAAGCTTGCGTCCCTGTGGGGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                    <  1:641696/90‑1 (MQ=255)
                                                    ttGATCAAGCTTGCGTCCCTGTGGTGACAGATAAACCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCaa                                   <  2:26233/90‑1 (MQ=255)
                                                           aGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGc                            <  1:391057/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCTTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  1:780711/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  2:172636/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  2:538149/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGAAAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  2:766440/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGAAAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  1:43715/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGAAAAACCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  1:467466/90‑1 (MQ=255)
                                                             cTTGCGCCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  1:480692/90‑1 (MQ=255)
                                                              ttGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAcc                         <  1:271559/90‑1 (MQ=255)
                                                                gCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcat                       >  1:1206674/1‑90 (MQ=255)
                                                                  gTCCTTGTGGTGAAAGATAAACCCCCTTGCCCCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcatca                     <  1:584386/90‑1 (MQ=255)
                                                                     ccTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTa                  <  2:84698/90‑1 (MQ=255)
                                                                       tgtgGTGACAGATAAACCCCCTTTGCCCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAAc                <  1:1116482/90‑1 (MQ=255)
                                                                          ggtgACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATc             >  1:245418/1‑90 (MQ=255)
                                                                             gACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATcccc          >  1:1083891/1‑90 (MQ=255)
                                                                               cAGATAAACCCCCTTTGCGCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCgg        <  1:694342/90‑1 (MQ=255)
                                                                                  aTAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTcc     >  2:161502/1‑90 (MQ=255)
                                                                                    aaaTCACTTTGGCGCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgc   <  2:1206674/90‑1 (MQ=255)
                                                                                     aaTCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgcc  <  2:837336/90‑1 (MQ=255)
                                                                                     aaTCCCCTTTGCCCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgcc  <  2:1103228/90‑1 (MQ=255)
                                                                                         |                                                                                     
GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCC  >  NZ_CP009273/2738359‑2738533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: