Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,738,4620AC54.0% 30.4 / 43.8 37G79G (GGT→GGGtrmDtRNA (guanosine(37)‑N1)‑methyltransferase TrmD
Reads supporting (aligned to +/- strand):  ref base A (11/6);  new base C (0/20);  total (11/26)
Fisher's exact test for biased strand distribution p-value = 1.45e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.97e-04
Rejected as consensus: Frequency below/above cutoff threshold.

ATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATC  >  NZ_CP009273/2738373‑2738548
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aTCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCa                                                                                        >  2:1220995/1‑90 (MQ=255)
   attttttGATTCGTTGCCGTTTCGCTGACGCCCGCTTGATAAAGCTTGGGTCCGTGTGGTGAAAGATACTTCACCTTTGCGCTTTCcccc                                                                                     <  1:1220995/88‑1 (MQ=255)
   aaTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCAccc                                                                                     >  1:766440/1‑90 (MQ=255)
       tttGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCccgccg                                                                                 <  2:786720/90‑1 (MQ=255)
         tGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCcgcg                                                                               <  2:393976/90‑1 (MQ=255)
         tGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCcgcg                                                                               >  2:1162825/1‑90 (MQ=255)
              cGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCttt                                                                          >  1:309665/1‑90 (MQ=255)
               tttcccAGTTCGCTGCCGCCCCCTTAATCACGCTTGCCTCCCTGTGGTGACAGAAAAATCACCTTTGCGCCTTCCCCCGCCGCGGCtttt                                                                         <  1:973951/86‑1 (MQ=255)
                  gCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTtgct                                                                      <  2:309665/90‑1 (MQ=255)
                  gCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTtgct                                                                      <  2:392628/90‑1 (MQ=255)
                      gTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCAt                                                                  >  1:319204/1‑90 (MQ=255)
                       ttCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATg                                                                 <  2:515021/90‑1 (MQ=255)
                            cgACGCCCGCTTGATCAAGCTTGCGTCCTTGTGGGGACAGAAAAATCCCCTTTGCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATgg                                                            <  1:901785/89‑1 (MQ=255)
                                gCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTc                                                        <  1:1135806/90‑1 (MQ=255)
                                     cTTGATCAAGCTTGCGTCCCTGTGGGGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                                   <  1:641696/90‑1 (MQ=255)
                                      ttGATCAAGCTTGCGTCCCTGTGGTGACAGATAAACCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCaa                                                  <  2:26233/90‑1 (MQ=255)
                                             aGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGc                                           <  1:391057/90‑1 (MQ=255)
                                               cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCTTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                                         <  1:780711/90‑1 (MQ=255)
                                               cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                                         <  2:172636/90‑1 (MQ=255)
                                               cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                                         <  2:538149/90‑1 (MQ=255)
                                               cTTGCGTCCCTGTGGTGACAGAAAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                                         <  2:766440/90‑1 (MQ=255)
                                               cTTGCGTCCCTGTGGTGACAGAAAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                                         <  1:43715/90‑1 (MQ=255)
                                               cTTGCGTCCCTGTGGTGACAGAAAAACCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                                         <  1:467466/90‑1 (MQ=255)
                                               cTTGCGCCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                                         <  1:480692/90‑1 (MQ=255)
                                                ttGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAcc                                        <  1:271559/90‑1 (MQ=255)
                                                  gCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcat                                      >  1:1206674/1‑90 (MQ=255)
                                                    gTCCTTGTGGTGAAAGATAAACCCCCTTGCCCCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcatca                                    <  1:584386/90‑1 (MQ=255)
                                                       ccTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTa                                 <  2:84698/90‑1 (MQ=255)
                                                         tgtgGTGACAGATAAACCCCCTTTGCCCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAAc                               <  1:1116482/90‑1 (MQ=255)
                                                            ggtgACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATc                            >  1:245418/1‑90 (MQ=255)
                                                               gACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATcccc                         >  1:1083891/1‑90 (MQ=255)
                                                                 cAGATAAACCCCCTTTGCGCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCgg                       <  1:694342/90‑1 (MQ=255)
                                                                    aTAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTcc                    >  2:161502/1‑90 (MQ=255)
                                                                      aaaTCACTTTGGCGCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgc                  <  2:1206674/90‑1 (MQ=255)
                                                                       aaTCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgcc                 <  2:837336/90‑1 (MQ=255)
                                                                       aaTCCCCTTTGCCCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgcc                 <  2:1103228/90‑1 (MQ=255)
                                                                               ttGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAg         >  1:967297/1‑90 (MQ=255)
                                                                                 gcgcCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGa       >  2:284988/1‑90 (MQ=255)
                                                                                      ttCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATc  >  2:1168807/1‑90 (MQ=255)
                                                                                         |                                                                                      
ATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATC  >  NZ_CP009273/2738373‑2738548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: