| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,957 | 0 | A | T | 53.3% | 8.3 / 33.4 | 30 | I17F (ATT→TTT) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base A (4/10); new base T (16/0); total (20/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGC > NZ_CP009273/3175874‑3176044 | tCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGTTTTTTTgcg > 1:511680/1‑90 (MQ=255) tttATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGGCCCGGTTTTTTGCGCGGTTTTCGGC‑GGtct‑cc > 1:621959/1‑89 (MQ=255) tataAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCGTTTTTTGGCGCGGTTTTCGGC‑GGTCT‑Ccgg > 2:108962/1‑90 (MQ=255) ataAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑CGGGc < 1:845183/90‑1 (MQ=255) ggAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑CGGGCaaaa < 1:108962/90‑1 (MQ=255) gAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGTTTTTTTGGGCGGTTTTCGGC‑GGTCTCCGGGCaaaa > 2:1087276/1‑90 (MQ=255) tGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑CGGGCAAAAACCCt < 1:880669/90‑1 (MQ=255) tGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGGTTTTTTGGGCGGTTTTCGGC‑GTTTT‑CCGGCAAAAAaccc > 2:573125/1‑89 (MQ=255) aTGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑CGGGCAAAAACCCTCGAAc < 1:317837/90‑1 (MQ=255) tCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGGCACGTTTTTTTGGGCGTTTTTCGGC‑GTTTT‑CGGGCAAAAACCCCCCAAcccc > 2:713762/1‑88 (MQ=255) cAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGGCACGTTTTTTTGGGCGTTTTTCCGC‑GGTCT‑CCGGCAAAAAACCTCCAAccccc > 2:630345/1‑87 (MQ=255) gTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTGGGGCGTTTTTCGGC‑GTTCT‑CGGGCAAAAAACCTCGAACCCCTTa > 2:434531/1‑90 (MQ=255) ctctCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTgg < 2:1218748/90‑1 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGGGCCCCCTTTTTTGGTGCGTTTTTTCGC‑GTTCT‑CCGGGAAAAAACCCCGAACCCCCTTCTGGc > 1:1218748/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGAGCCCCGTTTTTTTGGGCGGTTTTCTCC‑GGTTT‑CGGGGGAAAAACCCCGAACCGCTTACttgg > 1:821605/1‑87 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTGGCGCGTTTTTCGGC‑GTTCT‑CGGGCAAAAAACCCCGAACCGCTTACTGGc > 2:1222191/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTTGGGCGTTTCTCGGC‑GTTTT‑CCGGCAAAAAACCTCGAACCGCTTACTggg > 2:467312/1‑89 (MQ=255) tcATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGt < 2:821605/90‑1 (MQ=255) cATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTTGGGCGTTTCTCCGC‑GTTCT‑CGGGCAAAAAACCTCGAACCCCTTTCTGGCggt > 2:638169/1‑88 (MQ=255) ttCTGACCATACTGGCGGGGGGAGCCCCGTTTTTTTGCGCGTTTTTCGGC‑GTTCT‑CGGGCAAAAAACCCCCAACCCCCTTCTGGCGtttt > 1:17728/1‑90 (MQ=255) gACCATACTGGCGGGGGGAGACACGGTTTTTTGCGCGTTTTCCGGC‑GTTTT‑CGGGCAAAAAACCTCCAAACGCTTATTTGCGGTTTctct > 2:151566/1‑90 (MQ=255) gACCATACTGGCGGGGGCGGCCACGGTTTTTTGCGGGGTTCTTGGC‑GTTTT‑CGGGCCAAAAACCCCCAACCCCTTTTTTGGGTTTTTGCt > 1:412269/1‑90 (MQ=255) gACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTTGCt > 2:310086/1‑90 (MQ=255) aTACTGGCGGGGGCAGCCACGGTTATTTGCGGGGTTTTCGGCGGTTTT‑CGGCAAAAAACCCCCAAACC‑CTTTCTTGCGGTTTTGCTAGGt > 2:727691/1‑90 (MQ=255) gCGGGGGCAGCCACGTTTTTTGGCGCGTTTCTCGGC‑GTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTtcg > 2:378318/1‑90 (MQ=255) gCGGGGGCAGCCACGGTTTTTTGGGCGGTTTTCGGC‑GTTTT‑CCGGCAAAAAACCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcg > 2:549428/1‑90 (MQ=255) gCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCgggg < 1:713762/90‑1 (MQ=255) gCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCgggg < 1:12458/90‑1 (MQ=255) gTTTATTGGCGCGTTTCTCGGC‑GTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGtt < 2:61171/90‑1 (MQ=255) ttATTGGCGCGTTTCTCGGC‑GTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGc < 2:17728/90‑1 (MQ=255) | TCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGC > NZ_CP009273/3175874‑3176044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |