| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,153,677 | 0 | T | G | 61.5% | 7.6 / 10.8 | 15 | V604G (GTG→GGG) | mdtC | multidrug efflux RND transporter permease subunit MdtC |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (8/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGC > NZ_CP009273/2153604‑2153759 | cAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGatgatg < 1:47396/90‑1 (MQ=255) tttCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTAt < 2:20082/90‑1 (MQ=255) ttCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATc < 2:336133/90‑1 (MQ=255) aTCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGGGAACAGCGGGGTGATGTTTATCACCCTCAAGccc > 2:142255/1‑89 (MQ=255) gTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTTGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCCcgcg > 1:317040/1‑90 (MQ=255) aTCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAAc < 2:24424/90‑1 (MQ=255) ccGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGGGAACAGCGGGGTGATGTTTTTCACCCTCAAACCACGCGGCGAACGc > 2:10608/1‑90 (MQ=255) tCACCGGCTTTACAGGCGGTTCGCGAGGGAACAGCGGGGTGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAAAGGCgcagc > 1:346219/1‑90 (MQ=255) tCACCGGCTTTACAGGCGGTTCGCGAGGGAACAGCGGGGAGATGTTTATCACCCCCAAGCCACCCGGCGAACGCAGCGAAAAGGcgccgc > 2:427081/1‑90 (MQ=255) aCCGGCTTTACAGGCGGTTCGGGAGGGAACAGCGGGGAGATGGTTTTCAACCTCAAACCCCGGGACGAACGCAGCGAAAAGGCGGAGGaa > 1:55467/1‑90 (MQ=255) aCCGGCTTTACAGGCGGTTCGCGAGGGGAAAGCGGGGAGATGTTTTTCTCCCCCCAACCCCCCGGCGAACGCAGCGAAAAGGCGCCGCaa > 2:444780/1‑90 (MQ=255) aCCGGCTTTACAGGCGGTTCGCGAGGGAACAGCGGGATGATGTTTTTCACCCCCAAGCCACCCGACGAACGCAGCGAAACGGCGCAGCaa > 2:427566/1‑90 (MQ=255) cGGTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCt > 1:74892/1‑90 (MQ=255) ggTTCGCGAGAGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTg < 2:211801/90‑1 (MQ=255) tCGCGAGGGAACAGCGGGGTGATGTTTATCACCCCCAAGCCACGCGGCGAACGCAGCGAAACGGCGCAGCCAATTATCGACCGTCTgcgc > 2:261338/1‑90 (MQ=255) | CAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGC > NZ_CP009273/2153604‑2153759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |