Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,290,2430TG57.1% 10.4 / 16.4 14L87F (TTA→TTC) ccmBheme exporter protein CcmB
Reads supporting (aligned to +/- strand):  ref base T (1/5);  new base G (8/0);  total (9/5)
Fisher's exact test for biased strand distribution p-value = 3.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03
Rejected as consensus: Frequency below/above cutoff threshold.

GCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAA  >  NZ_CP009273/2290167‑2290317
                                                                            |                                                                          
gCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCGAAGGGAACGGcaacaaaa                                                               >  2:91920/1‑88 (MQ=255)
     aGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAAt                                                          <  1:253960/90‑1 (MQ=255)
                     gACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCAACAAAATCAATTGTTTCAAAATGCCGt                                          >  2:135666/1‑90 (MQ=255)
                                       gcgcCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTa                                                                           >  1:402837/1‑39 (MQ=255)
                                       gcgcCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTa                                                                           <  2:402837/39‑1 (MQ=255)
                                       gcgcCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAAACGTCACGGa                        >  1:415210/1‑90 (MQ=255)
                                       gcgcCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAAAGGCAAGAACAACAATTTTTCAAGACGGCCGGCCTGCCAATCGTCTCggt                        >  1:253387/1‑89 (MQ=255)
                                       gcgcCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAAAGGCAACAACATCAATTGTTCAAAACTGCCGTCCCGCAAAACGTCACGGa                        >  1:455697/1‑90 (MQ=255)
                                        cgcCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGaa                       <  2:73945/90‑1 (MQ=255)
                                          ccATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCAACAACAACAATTTTTCAAAACTGCCGGCCTGCAAAACGTCACGGaaaa                     >  2:341087/1‑88 (MQ=255)
                                               aCCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCAACAACATCAATTTTTCAAGACCGCCGTCCTGCAAAACGTCACCGAACAGTCGt                >  2:84250/1‑90 (MQ=255)
                                                         gCACAACGGCGGGCAAGGGGAACCGCAACAACAACAATTGTTCAAAACTGCCCCCCTGCAAAAAGTCCCCGAACAGTCGTTCCAGCGCCa      >  2:379881/1‑90 (MQ=255)
                                                             aaCGGCGGGCAAGGGTAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCaa  <  1:113105/90‑1 (MQ=255)
                                                             aaCGGCGGGCAAGGGTAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCaa  <  1:341087/90‑1 (MQ=255)
                                                                            |                                                                          
GCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAA  >  NZ_CP009273/2290167‑2290317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑