| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,343,100 | 0 | A | C | 57.1% | 2.5 / 10.2 | 14 | pseudogene (36/105 nt) | BW25113_RS26180 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base C (0/8); total (3/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.49e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTAATGAAACGTGTCGTATTAATGAATTTCTTTTGCCACTACTAGCTTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATCAGGCGGATACAGCGATGAATTTTATTCGACAGGGAT > NZ_CP009273/2343013‑2343187 | ttAATGAAACGTGTCGTATTAATGAATTTCTTTTGCCACTACTAGCTTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTccacc > 1:344249/1‑90 (MQ=255) ccGTGTCGTATTAATGAATTTTTTTTCCCACTACTAGCTTGACCCCGCTTTTACCCTTCATTTGCAGCCCATAGATCCCCCCAGccacta < 2:203463/89‑1 (MQ=255) aCGTGTCGTATTAATGAATTTCTTTTGCCACTACTAGCTTGACACCGCTTTTCCCCTTCATTTGCAGCGCATAGATTCCCCCAGccacta < 2:190344/90‑1 (MQ=255) gtgtCGTATTAATGAATTTCTTTTGCCACTACTAGCTTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGccactacc < 1:263029/90‑1 (MQ=255) gatttttttttGCCACTACTAGCTTGCCCCCGCTTTTCCCCTTCATTTGCAGCGCATAGATCCCCCCAGCCACTACCACTAATAGTgcgc < 2:344249/87‑1 (MQ=255) gACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAAt > 1:37782/1‑90 (MQ=255) gACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAAt > 2:333306/1‑90 (MQ=255) gCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGccac < 1:155151/42‑1 (MQ=255) gCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGccac > 2:155151/1‑42 (MQ=255) tAGATTCCCCCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATCAGGCGGATACAGCGATGAATTTTATTCGAc < 1:117702/90‑1 (MQ=255) aGATTCCCCCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATCAGGCGGATACAGCGATGAATTTTATTCGACa < 1:83998/90‑1 (MQ=255) aGATTCCCCCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATCAGGCGGATACAGCGATGAATTTTATTCGACa < 2:319409/90‑1 (MQ=255) aGATTCCCCCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATCAGGCGGATACAGCGATGAATTTTATTCGACa < 2:92944/90‑1 (MQ=255) aGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATCAGGCGGATACAGCGATGAATTTTATTCGACa < 2:371083/90‑1 (MQ=255) aTTCCCCCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATCAGGCGGATACAGCGATGAATTTTATTCGACAgg < 1:333306/90‑1 (MQ=255) ccaccaGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATCAGGCGGATACAGCGATGAATTTTATTCGACAGGGAt > 2:365148/1‑90 (MQ=255) | TTAATGAAACGTGTCGTATTAATGAATTTCTTTTGCCACTACTAGCTTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATCAGGCGGATACAGCGATGAATTTTATTCGACAGGGAT > NZ_CP009273/2343013‑2343187 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |