Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,055,6790AC58.3% 7.2 / 10.9 12T491P (ACC→CCC) scpAmethylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base C (0/7);  total (3/9)
Fisher's exact test for biased strand distribution p-value = 4.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCAC  >  NZ_CP009273/3055599‑3055733
                                                                                |                                                      
aaGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGtgatg                                               >  2:192728/1‑90 (MQ=255)
                        tCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGa                       <  1:192728/90‑1 (MQ=255)
                          caCAACGTGATGGTGGGTACCGCGCAATTTCTTCCGCTGGAACCCTTTCGCGCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAAc                     <  1:258753/89‑1 (MQ=255)
                              cggTGATGGTGCGAACCGCGAAATTTGCTCCGCGGGACCCCATCCGCCCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGccc                 <  2:204147/88‑1 (MQ=255)
                                   aTGGTGCGTAACGAGAAAATTGCTCCGCTGGACCGCTTTCGCCCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACt            <  1:350055/90‑1 (MQ=255)
                                     ggTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAAc                     <  1:63394/79‑1 (MQ=255)
                                     ggTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAAc                     >  2:63394/1‑79 (MQ=255)
                                      gggCGTAACGAGCAAATTGTTTCGCTGGAACCCATTCGCCCCCCCGGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAc         <  1:304053/88‑1 (MQ=255)
                                      gTGCGAAACGACAAAATTGCTCCCCGGGAACGCTTCCGCCCCCCCCGTGAGGAGGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAc         <  2:160663/90‑1 (MQ=255)
                                        gCGTAACGCGCAAATTGCTTCGCTGGAACGCATCCGCCCCCCCCGTGAGGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAcgc       <  1:335319/90‑1 (MQ=255)
                                        gCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAcgc       >  2:26654/1‑90 (MQ=255)
                                             gcGAGCAAATTGCTCCGGGGGAACGCGTCCGCCCCCCCCGTGATGAGGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGcac  <  2:116376/89‑1 (MQ=255)
                                                                                |                                                      
AAGACGAAACCGATGTACTTGAGATCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTCGCTGGAACGCATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCAC  >  NZ_CP009273/3055599‑3055733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑