Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,294,1540GT54.5% 3.7 / 14.6 11T161N (ACC→AAC) ubiTSCP2 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (5/0);  new base T (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCC  >  NZ_CP009273/3294080‑3294225
                                                                          |                                                                       
cccGGCGCATCAATGGGATTTTCTCCTCGAATTAGCATGGTTCCCCTCCCGATGTCTGTTTGTTTTCGGGCGGGTTTTTCATCCCCGCCt                                                          <  2:389452/90‑1 (MQ=255)
  cGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTca                                                        >  1:294753/1‑90 (MQ=255)
    gcgcATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTcaac                                                      >  1:255336/1‑90 (MQ=255)
                      cTACCCAAATTAGCATGTTCCCCCTCCCGATGTTTTTTTGTTTTCGGGCGGGTTTTTCTTTCCCGCCTCAACAAAATCCGCCAGTTgcag                                    <  2:434840/90‑1 (MQ=255)
                                       gTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAgg                   >  2:399555/1‑90 (MQ=255)
                                              tACCGATTTCTTTTTGGTTTCAGGCGCGTTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCgg            <  1:440807/90‑1 (MQ=255)
                                                 cGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCAt         >  1:44970/1‑90 (MQ=255)
                                                        tttttGTTTTCAGGCGCGTTTTTCATCCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTcc  <  1:358770/88‑1 (MQ=255)
                                                        tgtttgttTTCAGGCGGGTTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTcc  <  1:372888/90‑1 (MQ=255)
                                                                       gcgTTTTTCATCCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTcc  <  2:421020/75‑1 (MQ=255)
                                                                       gcgGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTcc  >  1:421020/1‑75 (MQ=255)
                                                                          |                                                                       
CCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCC  >  NZ_CP009273/3294080‑3294225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑