| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,578,446 | 0 | A | C | 56.2% | 5.4 / 18.8 | 16 | Y580D (TAC→GAC) | ggt | gamma‑glutamyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (6/1); new base C (0/9); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTT > NZ_CP009273/3578359‑3578523 | cTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAcc > 1:263508/1‑90 (MQ=255) tACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAccc > 2:439854/1‑90 (MQ=255) aGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccgc > 2:355790/1‑90 (MQ=255) aCGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTaa > 1:188435/1‑90 (MQ=255) aaGGCTCCCTTCGGCTCCCCCAAAAAAAACCCCCCCCCCCCCACGAAGGGGGCCGCTACCCTTAGCCCCCCGCCGTTAAATCATCCACCg < 2:168375/90‑1 (MQ=255) cGGTTTCCCCTAAAAAAATCCCCCTCTCCCCACGAAGGGGCCCGCTAACCTTAGCCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCg < 1:355790/90‑1 (MQ=255) tccccTAAAAAAATCCCCCTCTCCCCACGAAGGGGCCCGCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgc < 1:439854/88‑1 (MQ=255) cccTGAAAAATTACCCCTTTCCCCAGAAAGGGGGCGGCTATCCTTAGGCCCCCGCCGTTAAATCATCCCCCGAGCGCGGGTCGGATgcgc < 1:249447/90‑1 (MQ=255) aaaaaaTACCCCTCCCCCCACGAAGAGGCCCGCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTAc < 1:22006/88‑1 (MQ=255) aaaaaTCCCCCCCTCCCCAGGAAGGGGCCCCCTACCCTTACCCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACa < 2:148694/89‑1 (MQ=255) ctccccACGAAGGGGCCCGCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCg < 1:143415/87‑1 (MQ=255) cACGAAGGGGGCCGTTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCa < 1:157630/90‑1 (MQ=255) gggcccGCTACCCTTCGCCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATaa < 2:209469/86‑1 (MQ=255) gggCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgc < 1:111215/57‑1 (MQ=255) gggCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgc > 2:111215/1‑57 (MQ=255) gCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCttt > 2:179155/1‑90 (MQ=255) | CTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTT > NZ_CP009273/3578359‑3578523 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |